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ProteomicsProteome-wide evaluation of plasma depletion methods vis-à-vis fractionation techniques
Human plasma is one of the most widely used tissues in clinical analysis, and plasma-based biomarkers are used for monitoring patient health status and/or response to medical treatment to avoid unnecessary invasive biopsy. Data driven plasma proteomics has suffered from a lack of throughput and dete...
ORGANISM(S): Homo sapiens (Human) 
2021-02-17 | PXD022469 | Pride
Electrophoresis Plasma Chromatography Mass spectrometry Prefractionation techniques Label-free quantitation Biomarkers Proteome 
ProteomicsAmmonium Sulfate-based Prefractionation Improved Proteome Coverage in Arabidopsis thaliana Leaf Extract
The hypothesis that ammonium sulfate-based protein precipitation can help prefractionate the total protein extract into fractions of reduced complexity and improve the detection of low-abundance proteins was investigated. This dataset resulted from the experiments that we performed, of which the res...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2022-02-25 | MSV000088920 | MassIVE
ProteomicsAmmonium Sulfate-based Prefractionation Improved Carbonylated Proteome Coverage in Arabidopsis thaliana Leaf Extract
The hypothesis that ammonium sulfate-based protein precipitation can help prefractionate the total protein extract into fractions of reduced complexity and improve the detection of low-abundance proteins was investigated. This dataset resulted from the experiments that we performed to improve the de...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2022-10-20 | MSV000090559 | MassIVE
ProteomicsProteome-wide evaluation of plasma depletion methods vis-à-vis fractionation techniques
Human plasma is one of the most widely used tissues in clinical analysis, and plasma-based biomarkers are used for monitoring patient health status and/or response to medical treatment to avoid unnecessary invasive biopsy. Data driven plasma proteomics has suffered from a lack of throughput and dete...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-06-25 | MSV000098333 | MassIVE
ProteomicsK562 cell lysates prefractionated by various methods
K562 cell lysates were prefractionated by protein reversed-phase chromatography (Prot-RP), SDS-PAGE, and high-pH peptide RP (Pept-RP) to compare their method characteristics. The starting material was prefractionated to 10 fractions for each method. Each prefractionation method was conducted in trip...
ORGANISM(S): Homo Sapiens (human) 
2024-10-15 | PXD049312 | JPOST Repository
ProteomicsPEPPI-SP3: Robust and Simple Sample Prefractionation Workflow for Top-Down Proteomics
Precise prefractionation of proteome samples is a potent method for realizing in depth analysis in top-down proteomics. PEPPI MS (Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS), a gel-based sample fractionation method, enables high-resolution proteome fractionation bas...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000095992 | MassIVE
ProteomicsPEPPI-SP3: Robust and Simple Sample Prefractionation Workflow for Top-Down Proteomics
In this study, we developed a complete, robust, and simple sample preparation workflow named PEPPI-SP3 for top-down proteomics by combining PEPPI-MS with the magnetic bead-based protein purification approach used in SP3 (single-pot, solid-phase-enhanced sample preparation), now one of the standard s...
ORGANISM(S): Homo Sapiens (human) 
2025-01-23 | PXD056413 | JPOST Repository
ProteomicsComprehensive O-Phosphoproteomic Landscape of Corynebacterium glutamicum
In this study, we implemented a highly robust workflow for O-phosphopeptides enrichment utilizing titanium-based immobilized metal affinity chromatography (Ti-IMAC). By integrating an offline reversed-phase prefractionation strategy and a combination of Electron-Transfer/Higher-Energy Collision Diss...
ORGANISM(S): Corynebacterium Glutamicum Atcc 13032 
2026-04-09 | PXD076840 |
UnknownOver 4100 protein identifications from a Xenopus laevis fertilized egg digest using reversed-phase chromatographic prefractionation followed by capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry analysis.
Not available
S-EPMC5140748 | biostudies-literature
OtherRapid Analyses of Proteomes and Interactomes Using an Integrated Solid-Phase Extraction-Liquid Chromatography-MS/MS System.
Data from ProteomeXchange, PXD ID: PXD001111. Experiment: NYLON_Hela, file: OR7_20130627_NB_Nylon_Hela_1ug_top20_1.mzml. Published as part of J Proteome Res. 2014 Dec 8 . From the Abstract: {{i}} Here, we explore applications of a LC system using disposable solid-phase extraction (SPE) cartridges a...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32310005326 | GPMDB
Resource Reanalysis 
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