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ProteomicsConditions for maintenance of hepatocyte differentiation and function in 3D cultures
3D spheroid cultures of primary human hepatocytes (PHH) are used in studies of hepatic drug metabolism and toxicity. However, the 3D spheroids are maintained under different conditions, with possible cofounding results. Here we performed an in-depth analysis of how various culture conditions influen...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD024632 | Pride
Primary human hepatocyte culture Lc-ms/ms 
TranscriptomicsA Comparative Study of Genome Wide Transcriptional Profiles of Primary Hepatocyte in In Vitro Cultures
The liver is one of most important organs in our bodies. It performs many essential functions including metabolism, synthesis, secretion, detoxification, and storage. Hepatocytes are the principal cell type in the liver and are involved in multiple liver-specific functions. There have been several e...
ORGANISM(S): Rattus norvegicus 
2010-06-08 | E-GEOD-20659 | biostudies-arrayexpress
TranscriptomicsAtorvastatin, rosuvastatin and rifampicin effect on human primary hepatocyte transcriptome
This SuperSeries is composed of the following subset Series: GSE24186: Atorvastatin, rosuvastatin and rifampicin effect on human primary hepatocyte transcriptome [Steroltalk platform] GSE24187: Atorvastatin, rosuvastatin and rifampicin effect on human primary hepatocyte transcriptome [Affymetrix pla...
ORGANISM(S): Homo sapiens 
2011-09-04 | E-GEOD-24188 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human primary hepatocytes treated with transcription factor PPAR agonist Wy14643
Studies in mice have shown that PPARalpha is an important regulator of hepatic lipid metabolism and the acute phase response. However, little information is available on the role of PPARalpha in human liver. Here we set out to compare the function of PPARalpha in mouse and human hepatocytes via anal...
ORGANISM(S): Homo sapiens 
2009-09-25 | E-GEOD-17251 | biostudies-arrayexpress
ProteomicsAnimal product-free culture conditions for 3D primary human hepatocyte spheroids improve long-term viability and drug metabolism
3D cultures of primary human hepatocytes (3D PHH) are successfully used to reduce and replace the use of animal experiments in biomedical research. Yet, the initial formation of 3D PHH is highly dependent on the supplementation with fetal bovine serum (FBS). However, the molecular composition of FBS...
ORGANISM(S): Homo sapiens (Human) 
2026-01-23 | PXD056428 | Pride
Human Setum-free Fbs Liver 3d phh 
GenomicsChIP-sequencing of hepatocyte-like cells (HLCs) and primary human hepatocytes (PHHs)
ChIP-sequencing was performed to explore the difference between the epigenetic profiles of hepatocyte-like cells (HLCs) generated in vitro and primary human hepatocytes (PHHs). HLCs were differentiated from hIPSCs and hESCs for 30 days, as previously reported (Hannan et al, 2013). PHHs were purchase...
ORGANISM(S): Homo sapiens 
2022-08-11 | E-MTAB-10637 | biostudies-arrayexpress
TranscriptomicsIn vitro activation and reversion of human primary hepatic stellate cells
Liver fibrosis is characterized by the excessive formation and accumulation of matrix proteins as a result of wound healing in the liver. A main event during fibrogenesis is the activation of the liver resident quiescent hepatic stellate cell (qHSC). Recent studies suggest that reversion of the acti...
ORGANISM(S): Homo sapiens 
2015-08-12 | E-GEOD-68001 | biostudies-arrayexpress
TranscriptomicsAtorvastatin, rosuvastatin and rifampicin effect on human primary hepatocyte transcriptome [Steroltalk platform]
With particular emphasis on interactions between cholesterol homeostasis and drug metabolism we investigate the transcriptome of human primary hepatocytes treated by two commonly prescribed cholesterol lowering drugs atorvastatin and rosuvastatin and by rifampicin that serves as an outgroup as well ...
ORGANISM(S): Homo sapiens 
2011-09-04 | E-GEOD-24186 | biostudies-arrayexpress
ProteomicsHepatocellular Metabolic Profile: Understanding Post-Thawing Metabolic Shift in Primary Hepatocytes in vitro
Positive and negative-mode metabolomics data from a time-course experiment for unexposed cells during a 24-hour time course, investigating the changes in metabolism of primary human hepatocytes in vitro post-thawing.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-04-01 | MSV000097484 | MassIVE
TranscriptomicsTranscription profiling of rat primary hepatocyte in culture after low dose and short incubation time of cadmium in primary
The study was performed using primary rat hepatocyte in culture from 4 adult male Sprague-Dawley rats to investigate the changes in gene expression under low dose (4μM) and short exposure (3hrs) of cadmium chloride. By comparing the gene expression profiles of control and cadmium-treated cells, the...
ORGANISM(S): Rattus norvegicus 
2009-03-07 | E-GEOD-8013 | biostudies-arrayexpress
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