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Proteins co-localizing with RNA targets of interest in HeLa cells were identified using our recently optimized hybridization-proximity labeling approach (HyPro2) combined with label-free mass spectrometry. Briefly, fixed and permeabilized cells were hybridized with digoxigenin-labeled antisense olig...
ORGANISM(S): Homo sapiens (Human) 
2025-08-04 | PXD063191 | Pride
From a set of 15 standard, normalized or full-length cDNA libraries, we generated 139 007 3'- or 5'-end sequenced ESTs, representing more than one-third of the c. 385 000 publicly available Populus ESTs. Clustering and assembly of 107 519 3'-end ESTs resulted in 14 451 contigs and 20 560 singletons,...
ORGANISM(S): Populus trichocarpa x Populus deltoides 
To determine the downstream molecular mechanism of PRLX-93936, we performed systematic proteomic profiling of cells treated with PRLX-93936, erastin, or JWZ-8-103. Results include global proteome profiling, ubiquitylation proteomics, and proximity labeling proteomics enabling comparison of drug-indu...
ORGANISM(S): Homo sapiens (Human) 
2025-08-15 | PXD059137 | Pride
Transcriptional reprogramming forms a major part of a plant's response to pathogen infection. Many individual components and pathways operating during plant defense have been identified but our knowledge of how these different components interact is still rudimentary. We have generated a high-resolu...
ORGANISM(S): Arabidopsis thaliana 
A novel experimental design strategy (A Mead et al, in preparation), based on the principle of the M-bM-^@M-^\loop designM-bM-^@M-^], was developed to enable efficient extraction of information about key sample comparisons using a two-colour hybridisation experimental system. With 192 distinct sampl...
ORGANISM(S): Arabidopsis thaliana 
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