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MetabolomicsIntegrating two chromatographies in a co-fractionation mass spectrometry approach improved protein-metabolite interactions (PMIs) mapping capabilities and revealed novel protein-ligand pairings in Escherichia coli.
The protein-metabolite interactome remains understudied. We previously developed a co-fractionation mass-spectrometry-based approach called PROMIS to address this gap. PROMIS relies on size separation chromatography of protein-metabolite complexes from lysates, followed by proteomics and metabolomic...
2025-04-25 | MTBLS9098 | MetaboLights
ProteomicsDiscovery of Regulatory Complexes in Arabidopsis via Protein–Metabolite Mapping During Pseudomonas Infection
Protein–metabolite interactions (PMIs) are critical regulators of cellular processes, yet their roles in plant–pathogen interactions remain poorly understood. Here, we applied PROMIS (Protein–Metabolite Interactions using Size separation) to map the Arabidopsis interactome during infection with Pseu...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Pseudomonas syringae pv. tomato str. DC3000 Bacteria 
2026-05-05 | PXD069625 | Pride
Nata1 Promis Protein-metabolite interactions 
UnknownMechanistic dissection of diabetic retinopathy using the protein-metabolite interactome.
Not available
S-EPMC7843730 | biostudies-literature
UnknownRewiring of the protein-protein-metabolite interactome during the diauxic shift in yeast.
Not available
S-EPMC9569316 | biostudies-literature
UnknownSelf- and cross-attention accurately predicts metabolite-protein interactions.
Not available
S-EPMC9887643 | biostudies-literature
UnknownIIS--Integrated Interactome System: a web-based platform for the annotation, analysis and visualization of protein-metabolite-gene-drug interactions by integrating a variety of data sources and tools.
Not available
S-EPMC4065059 | biostudies-literature
UnknownmiRNA-protein-metabolite interaction network reveals the regulatory network and players of pregnancy regulation in dairy cows.
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S-EPMC11329941 | biostudies-literature
UnknownMPI-VGAE: protein-metabolite enzymatic reaction link learning by variational graph autoencoders.
Not available
S-EPMC10359079 | biostudies-literature
UnknownGlobal mapping of protein-metabolite interactions in Saccharomyces cerevisiae reveals that Ser-Leu dipeptide regulates phosphoglycerate kinase activity.
Not available
S-EPMC7876005 | biostudies-literature
ProteomicsNew insight into interactome of plant NDPKs
Interactions between metabolites and proteins play an integral role in all cellular functions. Here we describe an affinity-purification (AP) approach in combination with LC/MS-based metabolomics and proteomics that allows, to our knowledge for the first time, to analyse protein‒metabolite and prote...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-10-09 | PXD006294 | Pride
Metabolites Glutathionylation Affinity purification A. thaliana Ndpk Plant Protein‒metabolite interactions Protein‒protein interactions 
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