In this study, we applied the isobaric tags for relative and absolute quantitation (iTRAQ) technique to detect alterations in the proteomic profile of the jejunal mucosa using a porcine model in which piglets were offered the protein-limited (PL) diet. Protein identification and quantification for i...
Twenty million LbetaT2 cells were transfected with either control or Galphas siRNA, then were seeded in 100-mm cell culture plates in DMEM + 10% FBS. Two days later, cells were washed twice with pre-warmed PBS. Conditioned media was harvested another 24 h later, and centrifuged at 20,000 g for 10 mi...
To discover ESCC related proteins, we used SWATH to quantify the protein abundance between ESCC and adjacent tissues. Briefly, we pooled 10 ESCCtissues and their corresponding adjacent tissues for SWATH acquisition with three replicates.Three DDA repeats were also acquired with the pooled 10-paired ...
In the current study, we attempted to characterize the DNA repair system of Mycopalsma gallisepticum. We identified members of different DNA repair pathways based on genomic data and publications. We quantified mRNA of respective proteins per cell in a set of adverse conditions and identified most o...
Data provided is derived from our research on comparing protein expression profiling of Garcinia mangostana (G. mangostana) Linn. on different ripening stages. Proteins were extracted from the pericarp of G. mangostana at three points of ripening stages (stage 0, 2 and 6) and digested with trypsin b...
Using affinity chromatography to enrich carbonylated proteins from rice embryos germinated for 0, 24, 48, 72 h. Sequential window acquisition of all therotical spectra (SWATH) technology was used to quantify carbonylated proteins. Mass spectra data was searched to discover carbonylated peptides and ...
Long non-coding RNAs (lncRNA) have been identified as key regulators of tumorigenesis and development. We aim to explore the biological functions and molecular mechanisms of lncRNA MIR200CHG in breast cancer. We found that MIR200CHG is highly expressed in breast cancer tissues and is related to the ...
D13_1, and D13_2 are 8 plex iTRAQ analyses of depleted serum samples. The files are group files of ProteinPilot analyses of the collected SCX fractions .
Polyporus umbellatus sclerotia are medicinal tissue, but little is known about acetylome of sclerotia generated from hyphae. Acetylated peptides in P. umbellatus were enriched by anti-acetyl-lysine antibody beaded agarose and DDA data were acquired by AB Triple TOF 5600+. Raw data were analyzed by P...
