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ProteomicsProteomic analysis reveals the high specificity and lack of collateral activity of targeted PspCas13b
To gain a global view on the impact of the collateral activity on protein expression levels, mass spectrometry was used for proteomics analysis of HEK 293T cells when PspCas13b was co-expressed with non-targeted (NT) crRNA or targeting (T) crRNA, which targets the BCR-ABL1 mRNA breakpoint. There was...
ORGANISM(S): Homo sapiens (Human) 
2024-05-20 | PXD051089 | Pride
Hek293 t cells Pspcas13b Collateral activity 
UnknownSingle-base tiled screen unveils design principles of PspCas13b for potent and off-target-free RNA silencing.
Not available
S-EPMC11564092 | biostudies-literature
UnknownDeveloping PspCas13b-based enhanced RESCUE system, eRESCUE, with efficient RNA base editing.
Not available
S-EPMC8356384 | biostudies-literature
TranscriptomicsCharacterization of gRNA-Dependent and gRNA-Independent Off-Target Binding Sites of PspCas13b and RfxCas13d in Mammalian Cells [RNA-Seq]
CRISPR-Cas13 systems, harnessed for RNA-guided transcriptome editing, hold significant promise for clinical and in vivo therapeutic applications. However, understanding their in vivo target specificity and recognition rules remains a challenge. In this study, we employed the uSpyCLIP method, which e...
ORGANISM(S): Homo sapiens 
2026-01-27 | GSE291437 | GEO
OtherCharacterization of gRNA-Dependent and gRNA-Independent Off-Target Binding Sites of PspCas13b and RfxCas13d in Mammalian Cells [CLIP-seq]
CRISPR-Cas13 systems, harnessed for RNA-guided transcriptome editing, hold significant promise for clinical and in vivo therapeutic applications. However, understanding their in vivo target specificity and recognition rules remains a challenge. In this study, we employed the uSpyCLIP method, which e...
ORGANISM(S): Homo sapiens 
2026-01-27 | GSE291436 | GEO
GenomicsCharacterization of gRNA-Dependent and gRNA-Independent Off-Target Binding Sites of PspCas13b and RfxCas13d in Mammalian Cells [CLIP-seq]
Characterization of gRNA-Dependent and gRNA-Independent Off-Target Binding Sites of PspCas13b and RfxCas13d in Mammalian Cells [CLIP-seq]
PRJNA1233595 | ENA
ProteomicsSystematic silencing of oncogenic fusion transcripts with ultra-precise CRISPR-Cas13b
Oncogenic gene fusions are key drivers of cancer, yet most remain untargetable by current therapies. Here, we establish CRISPR-PspCas13b as a personalizable platform for systematic silencing of various fusion transcripts. We reveal that recognition and cleavage of the breakpoint sequence by PspCas13...
ORGANISM(S): Homo sapiens (Human) 
2026-08-26 | PXD060758 | Pride
GenomicsCharacterization of gRNA-Dependent and gRNA-Independent Off-Target Binding Sites of PspCas13b and RfxCas13d in Mammalian Cells [RNA-Seq]
Characterization of gRNA-Dependent and gRNA-Independent Off-Target Binding Sites of PspCas13b and RfxCas13d in Mammalian Cells [RNA-Seq]
PRJNA1233596 | ENA
TranscriptomicsMolecular principles of CRISPR-Cas13b design for selective silencing of oncogenic fusion transcripts
Oncogenic gene fusions are key drivers of cancer, yet most remain untargetable by current therapies. Here, we establish CRISPR-PspCas13b as a personalisable platform for systematic silencing of various fusion transcripts. We reveal that recognition and cleavage of the breakpoint sequence by PspCas13...
ORGANISM(S): Homo sapiens 
2026-08-26 | GSE321679 | GEO
ProteomicsMolecular principles of CRISPR-Cas13b design for selective silencing of oncogenic fusion transcripts
Oncogenic gene fusions are key drivers of cancer, yet most remain untargetable by current therapies. Here, we establish CRISPR-PspCas13b as a personalisable platform for systematic silencing of various fusion transcripts. We reveal that recognition and cleavage of the breakpoint sequence by PspCas13...
ORGANISM(S): Homo sapiens (Human) 
2026-08-26 | PXD074216 | Pride
Cas13 
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