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The non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi is investigated using the RNA-seq technology. A dedicated computational pipeline analyzes RNA-seq reads and prior genome annotation to identify small RNAs, untranslated regions of mRNAs, and cis-encoded antisense transc...
ORGANISM(S): Pyrococcus abyssi 
Origin of replication for Pyrococcus abyssi by ChIP-chip
RNA at 92 °C: the non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi.
Recombinant aRNase J from P. abyssi, with C-terminal (His)6-tag (aRNase J-His), was produced, purified and used as baits for AP-MS experiments. In order to discriminate specific protein interaction from column background, we performed mock AP-MS analyses with P. abyssi cell extracts in absence of pr...
ORGANISM(S): Pyrococcus abyssi 
2020-01-21 | PXD015856 | Pride
Hyperthermophilic archaeon
Recent advances in the study of archaeal DNA replication have uncovered defined replication origins (oriC) and demonstrated specific binding of the Cdc6/Orc1 protein and Mcm helicase to oriC in vivo and/or in vitro. The oriC of the hyperthermophilic archaeon Pyrococcus abyssi is characterized by 13 ...
ORGANISM(S): Pyrococcus abyssi 
2008-01-01 | GSE4104 | GEO
The non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi is investigated using the RNA-seq technology. A dedicated computational pipeline analyzes RNA-seq reads and prior genome annotation to identify small RNAs, untranslated regions of mRNAs, and cis-encoded antisense transc...
ORGANISM(S): Pyrococcus abyssi 
2014-05-31 | GSE58131 | GEO
Anaerobic hyperthermophilic archeon
Ribosome hibernation preserves translation machinery during stress, yet its mechanisms in Archaea remain poorly defined. Here, we identify HibA, a previously unrecognized family of hibernation factors widespread in Archaea. HibA consists of a bacterial-like HPF/RaiA domain fused to a Cystathionine B...
ORGANISM(S): Pyrococcus abyssi GE5 
2026-03-24 | PXD073243 | Pride
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