Sort   by:  
 Page size 
ProteomicsToll like receptor 2release by macrophages
THP-1 cells were cultured and EVCs were isolated after treatment with dexamethasone, LPS and a mixture of dexamethasone/LPS or non treated (control) by ultracentrifugation. Proteins were separated by 1D SDS-PAGE, tryptic digested and analyzed by nanoLC-MSMS
ORGANISM(S): Homo sapiens (Human) 
2019-08-13 | PXD013977 | Pride
Lcmsms human evc Thp-1 monocytes 
ProteomicsProtein partners identification in Pyrococcus abyssi by nanoLC-MSMS
Recombinant aRNase J from P. abyssi, with C-terminal (His)6-tag (aRNase J-His), was produced, purified and used as baits for AP-MS experiments. In order to discriminate specific protein interaction from column background, we performed mock AP-MS analyses with P. abyssi cell extracts in absence of pr...
ORGANISM(S): Pyrococcus abyssi 
2020-01-21 | PXD015856 | Pride
Pull-down Nanolc-msms Pyrococcus abyssi 
ProteomicsNanoLC-MSMS TMT comparison of circulated and unstimulated in vitro cultured reticulocytes
Triplicate samples of unstimulated in vitro culture-derived reticulocytes (derived from CD34+ cells) and cultured reticulocytes circulated overnight in an ex vivo circulation system were analysed through TMT-based quantitative proteomics to explore the impact of shear stress on reticulocyte protein ...
ORGANISM(S): Homo sapiens (Human) 
2018-08-03 | PXD009024 | Pride
Human Reticulocyte Nanolc-msms Erythrocyte Erythropoiesis 
ProteomicsNanoLC-MSMS TMT comparison of in vitro cultured/native reticulocytes and erythrocytes
Triplicate samples of donor-matched native reticulocytes (CD71+ cells), erythrocytes (CD71- cells) and in vitro culture-derived reticulocytes (derived from CD34+ cells) were analysed through TMT-based quantitative proteomics to examine possible protein abundance changes underlying reticulocyte matur...
ORGANISM(S): Homo sapiens (Human) 
2018-08-03 | PXD009023 | Pride
Human Reticulocyte Nanolc-msms Erythrocyte Erythropoiesis 
ProteomicsNanoLC-MSMS Phosphoproteomics comparison of in vitro cultured/native reticulocytes and erythrocytes
Triplicate samples of donor-matched native reticulocytes (CD71+ cells), erythrocytes (CD71- cells) and in vitro culture-derived reticulocytes (derived from CD34+ cells) were analysed through qualitative phosphoproteomics to examine possible novel mechanisms underlying reticulocyte maturation.
ORGANISM(S): Homo sapiens (Human) 
2018-08-03 | PXD009015 | Pride
Human Phospho Reticulocyte Nanolc-msms Erythrocyte Erythropoiesis 
ProteomicsMouse Articular chondrocyte LC-MSMS of wild-type, miR-26a -/- and cartilage-specific miR-23ab clusters knockout mice
To reveal the potential regulation target genes of miR-26a and miR-23a/b clusters in articular chondrocytes, we performed a multi-omics analysis of LC-MSMS and RNA-seq using cultured chondrocytes samples, which were primarily isolated from 3-week-old wild-type, miR-26a -/- (with or without miR-26a m...
ORGANISM(S): Mus musculus (Mouse) 
2023-01-25 | PXD031868 | Pride
Lc-msms Chondrocyte Mouse Mir-26a Mir-23 cluster 
ProteomicsNanoLC-MSMS Phosphoproteomics comparison of in vitro cultured reticulocytes before and after passage through microfluidic capillaries
Triplicate samples (biological replicates) of in vitro culture-derived reticulocytes (derived from CD34+ cells) were analysed through qualitative phosphoproteomics before and after passage through a microfluidics capillary chip to investigate the mechanisms underlying shear stress recognition.
ORGANISM(S): Homo sapiens (Human) 
2019-09-12 | PXD013652 | Pride
Human Red blood cell Phospho Reticulocyte Nanolc-msms Erythrocyte Erythropoiesis 
ProteomicsStructural mapping of the surfaces of fibrillar 1N3R and 1N4R human tau
The structural characterization of pathogenic tau filaments that accumulate in tauopathies, including Alzheimer disease, is key for understanding their pathogenic function and identify the structural determinants of distinct tau filaments involved in distinct tauopathies. Fibrillar tau surfaces play...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD027403 | Pride
Structural proteomics  proteolysis  chemical surface modification  nhs-biotin  nanolc-msms Tau fibrils 
ProteomicsNanoLC-MSMS TMT surfaceome comparison of ex vivo derived macrophage subtypes.
Triplicate samples of donor matched ex vivo generated macrophage subtypes M1, M2a, M2c compared against the M0 control analysed using TMT-based 11plex quantitative proteomics.
ORGANISM(S): Homo sapiens (Human) 
2023-09-04 | PXD032823 | Pride
Human Macrophage Polarisation 
ProteomicsNanoLC-MSMS TMT whole cell comparison of ex vivo derived macrophage subtypes.
Triplicate samples of donor matched ex vivo generated macrophage subtypes M1, M2a, M2c compared against the M0 control analysed using TMT-based 11plex quantitative proteomics.
ORGANISM(S): Homo sapiens (Human) 
2023-09-04 | PXD032801 | Pride
Human Macrophage Polarisation 
Sort   by:  
 Page size