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ProteomicsHuman urinary protein and glycopeotein LC-MSMS
N-glycosylation is one of the most common and important post-translational modifications, and plays a vital role in the control of many biological processes. The increasing discovery of abnormal alterations of N-linked glycans associated with many diseases leads to greater demands for rapid and effi...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD024639 | Pride
Lc-msms Urinary proteins 
ProteomicsCell Data-Indenpendent acquisition LC-MS/MS
In this work, a microwell-chip was prepared and modified. The microwell-chip was used for extraction of metabolites and subsequent protein digestion. Next, direct electrospray ionization mass spectrometry (ESI-MS) was adopted for metabolome identification and a data independent acquisition (DIA)-MS ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-11-08 | PXD025634 | Pride
Dia Cell Lc-ms/ms 
ProteomicsOGA-mediated protein glycosylation regulates pluripotency states in human embryonic stem cells
In our previous study, we uncovered that OGA significantly elevated in naïve hESC. Therefore, we sought to investigate the effects of O-GlcNAc on hESC and the interconvert of both pluripotent states. Depletion of OGA, which results in global O-GlcNAcylation increased, would impair naïve hESC pluripo...
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD041605 | Pride
Human embryonic stem cells，oga，o-glcnac 
ProteomicsAn integrated MS data processing strategy for fast identification, in-depth and reproducible quantification of protein O-glycosylation in large cohorts of human urine samples
Protein O-glycosylation has long been recognized to be closely associated with many diseases, particularly with tumor proliferation, invasion and metastasis. The ability to efficiently profile the variation of O-glycosylation in large-scale clinical samples provides an important approach for the devel...
ORGANISM(S): Homo sapiens (Human) 
2020-02-04 | PXD015987 | Pride
O-glycopeptide  urine  sialic acid 
ProteomicsLarge-scale identification of RNA-binding proteins
We developed a method, which enables unbiased, systematic identification of RBPs. Applying this method, we identified a large number of RBPs, adding hundreds of candidate RBPs to those previously known. In addition, we plotted the first large-scale distribution profile of RBPs after inhibition of RN...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD023162 | Pride
Rna-binding proteins(rbps)  lc-msms  large-scale 
ProteomicsAn Orthogonal Extraction Workflow for High-Depth Plasma Proteomics with Metabolomic Compatibility from Low Microliter-Scale Samples
Herein, we report an orthogonal extraction workflow that achieves enhanced proteomic profiling depth while preserving metabolomic compatibility from a mere 10 µL of plasma. We demonstrate that a ternary solvent mixture (TSM) of methanol, acetonitrile, and acetone exhibits distinct chemical orthogona...
ORGANISM(S): Homo Sapiens 
2026-02-03 | PXD073964 |
ProteomicsNovel molecular glue degraders against broad range of myeloma and lymphoma cancer cell lines
Targeted protein degradation of neosubstrates plays a crucial role in treating hematological cancers with IMiDs therapy. However, the inevitable drug resistance and hematological toxicities remain persistent challenges, limiting their clinical efficacy. Herein, we present the discovery of a lead deg...
ORGANISM(S): Homo Sapiens 
2024-06-22 | PXD053334 |
ProteomicsA Rapid and Sensitive Single-cell Proteomic Method Based on Fast LC Separation, Deep-learning RT Prediction and MS1-only Acquisition
We report an integrate strategy that combines fast liquid chromatography (LC) separation, deep learning-based retention time (RT) prediction and MS1-only acquisition for sensitive and rapid single-cell proteome analysis. Finally, SCP-MS1 was applied to sorafenib resistant HepG2 cells.
ORGANISM(S): Homo Sapiens 
2022-08-26 | PXD036211 |
ProteomicsDeep Profiling of the Human Plasma Proteome with Surface-Functionalized Molybdenum Disulfide Nanomaterials
Here, we report the synthesis and characterization of surface-functionalized magnetic molybdenum disulfide (MoS2) for the large-scale enrichment of low abundant plasma proteins and deep profiling by mass spectrometry.
ORGANISM(S): Homo Sapiens Escherichia Coli 
2022-09-15 | PXD036756 |
ProteomicsIdentification of N-Glycopeptides based on Glycocarrier Strategy
In this work, we developed a glycocarrier strategy to amplify MS signals with isobaric labeling for highly sensitive intact N-glycopeptide characterization from single and small numbers of cells without enrichment. Successful application in cell lines and microglia from the regional mouse brain demo...
ORGANISM(S): Homo Sapiens Mus Musculus 
2022-11-18 | PXD038212 |
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