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MetabolomicsComparison of singlequad and Orbitrap mass analyzer coupled to gas chromatography reveals potential of high resolution GC MS for untargeted metabolomics

The development of improved spectrometers and supporting computational tools is expected to eventually enable the rapid annotation of whole metabolomes. However, rarely are novel mass spectrometers directly compared to the previously used ones in the field of metabolomics, thus preventing researc...

2020-01-24 | MTBLS1104 | MetaboLights
ProteomicsIntensity-based label-free proteomics using a quadrupole orbitrap mass spectrometer
We present a unique data set for benchmarking label free quantitative proteomics using a quadrupole orbitrap mass spectrometer. Soluble Escherichia coli digest was spiked into a HeLa digest in four different concentrations, simulating protein expression differences. The data set, which is available ...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2015-03-25 | PXD001385 | Pride
Q exactive Quadrupole orbitrap Proteomics Label-free 
MetabolomicsAnnotation of the human adult urinary metabolome and metabolite identification using ultra high performance liquid chromatography coupled to a LTQ-Orbitrap mass spectrometer
This study deals with the annotation of the human adult urinary metabolome and metabolite identification from electrospray-mass spectrometry (ESI-MS) based metabolomics datasets. Metabolite identification was achieved using two complementary approaches: (i) formal identification by matching chromato...
2013-07-26 | MTBLS20 | MetaboLights
ProteomicsTriggered offset fragmentation allows massively multiplexed target profiling on quadrupole-orbitrap mass spectrometers
Parallel reaction monitoring (PRM) on quadrupole-orbitrap mass spectrometers has a limited multiplexing capacity which depends heavily on the reproducibility of peptide retention times. To overcome these limitations, we aimed to establish a data acquisition mode that allows retention-time-independen...
ORGANISM(S): Candida albicans (Yeast) Homo sapiens (Human) 
2020-06-19 | PXD017778 | Pride
Prm Multiplexing Targeted proteomics Quantification Lc-ms/ms 
ProteomicsThe Q Exactive HF, a Benchtop Mass Spectrometer with a Pre-filter, High Performance Quadrupole and an Ultra-High Field Orbitrap Analyzer
The quadrupole Orbitrap mass spectrometer (Q Exactive) has made a powerful proteomics instrument available in a benchtop format. It significantly boosted analyzable proteins per hour and has now evolved into a proteomics analysis workhorse for many laboratories. Here we describe the Q Exactive Plus ...
ORGANISM(S): Homo sapiens (Human) 
2014-11-10 | PXD001203 | Pride
Segmented quadrupole Q exactive hf Orbitrap ultra-high field Q exactive plus Quadrupole pre-filter Shotgun proteomics 
UnknownImproving the Quadrupole to Ion Mobility Region in a Digital Quadrupole/Ion Mobility/Orbitrap Mass Spectrometer.
Not available
S-EPMC12598848 | biostudies-literature
ProteomicsComplement Reporter Ion Quantification with a Quadrupole-Ion Trap Instrument
Proteomics workflows have traditionally been divided into discovery-based and targeted approaches, with instrumentation optimized for each. Discovery experiments typically utilize high-resolution analyzers such as the Orbitrap™, while targeted workflows rely on the sensitivity and specificity of tri...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) Xenopus laevis (African clawed frog) 
2025-09-08 | PXD062540 | Pride
Stellar Complement reporter ion Quadrupole-ion trap Multiplexed shotgun proteomics 
ProteomicsUltra-fast proteome analysis of HEK293T cells using Q-Orbitrap MS
We established an ultrafast proteomic method based on a 5-min gradient LC and quadrupole-Orbitrap mass spectrometer.
ORGANISM(S): Homo Sapiens (human) 
2022-07-28 | PXD034227 | JPOST Repository
UnknownCharacterization and Identification of Prenylated Flavonoids from Artocarpus heterophyllus Lam. Roots by Quadrupole Time-Of-Flight and Linear Trap Quadrupole Orbitrap Mass Spectrometry.
Not available
S-EPMC6943520 | biostudies-literature
ProteomicsA Compact Quadrupole-Orbitrap Mass Spectrometer with FAIMS Interface Improves Proteome Coverage in Short LC Gradients
State-of-the-art proteomics-grade mass spectrometers can measure peptide precursors and their fragments with ppm mass accuracy at sequencing speeds of tens of peptides per second with attomolar sensitivity. Here we describe a compact and robust quadrupole-orbitrap mass spectrometer equipped with a f...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2020-02-17 | PXD016662 | Pride
Faims Dia Tmt Bottom-up shotgun proteomics Phosphoproteomics Orbitrap Dda 
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