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Shotgun proteomics on sterile culture supernatant from S. aureus clinical isolates to determine proteins associated with pro-inflammatory phenotype.
ORGANISM(S): Staphylococcus aureus 
2022-02-22 | PXD028604 | Pride
A sulfur-containing analog of tryptophan, thiotryptophan (3-benzothienyl-L-alanine, S-Trp), was genetically incorporated into EcKatG to replace the MYW-forming tryptophan, W105. The S-Trp-bearing peptide was isolated using trypsin digestion followed by HPLC separation. Tandem mass spectrometry (MS/M...
ORGANISM(S): Escherichia coli 
2026-05-13 | PXD078006 | Pride
A sulfur-containing analog of tryptophan, thiotryptophan (3-benzothienyl-L-alanine, S-Trp), was genetically incorporated into EcKatG to replace the MYW-forming tryptophan, W105. The heme free apo-protein was purified, after heme-reconstitution and peroxide treatment, the S-Trp-bearing peptide was is...
ORGANISM(S): Escherichia coli 
2026-05-13 | PXD078069 | Pride
Usage of synonymous codons represents a characteristic pattern of preference in each organism. It has been inferred that such bias of codon usage has evolved as a result of adaptation for efficient synthesis of proteins. Here we examined synonymous codon usage in genes of the fission yeast Schizosac...
ORGANISM(S): Schizosaccharomyces pombe 
Actinic keratoses (AK) are premalignant lesions common on photo-damaged skin that, over time, can progress to squamous cell carcinoma (SCC). A high bacterial load of Staphylococcus aureus is associated with AK and SCC but it is unknown whether this has a direct impact on skin cancer development. To ...
ORGANISM(S): Homo sapiens (Human) 
2022-05-11 | PXD031622 | Pride
Noncoding expansions of a hexanucleotide repeat (GGGGCC) in the C9orf72 gene are the most common cause of familial amyotrophic lateral sclerosis and frontotemporal dementia. Here we report transgenic mice carrying a bacterial artificial chromosome (BAC) containing the full human C9orf72 gene with ei...
ORGANISM(S): Mus musculus 
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