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ProteomicsReimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries
Current methods for measuring amino-acid side-chain reactivity lack the throughput needed to screen large chemical libraries for interactions across the proteome. Here we redesigned the workflow for activity-based protein profiling of reactive cysteine residues by using a smaller desthiobiotin-based...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-01-26 | PXD022511 | Pride
Reactive cysteines Chemical proteomics 
TranscriptomicsTranscription profiling by array of human conjunctival epithelial side population cells
Side population (SP) cells isolated from limbal and conjunctival epithelia derive from cells that are slow cycling in vivo, a known feature of tissue stem cells. The purpose of this study was to define the molecular signature of the conjunctival side population cells by global differential gene expr...
ORGANISM(S): Homo sapiens 
2009-11-11 | E-GEOD-12631 | biostudies-arrayexpress
TranscriptomicsCAL-51 breast cancer side population cells
Human solid tumors contain rare cancer side population (SP) cells, which expel the fluorescencent dye H33342 and display cancer stem cell characteristics. Transcriptional profiling of cancer SP cells isolated by H33342 fluorescence analysis is a newly emerging approach to discover cancer stem cell m...
ORGANISM(S): Homo sapiens 
2009-12-31 | E-GEOD-18773 | biostudies-arrayexpress
UnknownReimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries.
Not available
S-EPMC8316984 | biostudies-literature
ProteomicsChemoproteomic profiling to identify activity changes and functional inhibitors of DNA-binding proteins
DNA-binding proteins are promising therapeutic targets but notoriously difficult to drug. Here, we evaluate a chemoproteomic DNA interaction platform as a complementary strategy for parallelized compound profiling. To enable this approach, we determined the proteomic binding landscape of 92 immobili...
ORGANISM(S): Homo sapiens (Human) 
2023-02-03 | PXD037297 | Pride
Reactive side chain profiling Mass spectrometry Drug screening Pull-down Covalency Dna binding Chemical proteomics 
TranscriptomicsTranscription profiling of pig endothelial cells from animials fed high or low cholesterol diet shows brief hypercholesterolemia induces side-specific protective changes in pig aortic valve endothelia
The study demonstrates (i) spatial heterogeneity of endothelial responses to hypercholesterolemia in the aortic valve, (ii) that endothelial metabolism is not dysfunctional in early focal lipid insudation, and (iii) that protective endothelial pathways induced by hypercholesterolemia may initially i...
ORGANISM(S): Sus scrofa 
2009-11-16 | E-CBIL-47 | biostudies-arrayexpress
TranscriptomicsEffects of dexamethasone on human skeletal muscle and adipose tissue
Context: Glucocorticoids are frequently prescribed drugs with important side-effects such as glucose intolerance and tissue remodelling. Objective: The goal was to explore the molecular basis of the crosstalk between adipose tissue and skeletal muscle during short term glucocorticoid treatment. Des...
ORGANISM(S): Homo sapiens 
2011-11-28 | E-GEOD-17992 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of endothelial cells from opposite sides of aortic valves from healthy adult male pigs
Differential transcriptional profiles from endothelial cells isolated from opposite sides of aortic valves from healthy adult male pigs (N=8, from slaughterhouse). Samples were hybridized to a human array and paired by animal.
ORGANISM(S): Sus scrofa 
2007-07-14 | E-CBIL-32 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of kidney samples from Wilms tumor patients to examine down- and up-regulation of genes either side of 1p13 breakpoints
Wilms tumour karyotypes frequently exhibit recurrent, large-scale chromosomal imbalances, among the most common of which are concurrent loss of 1p and gain of 1q. We have previously identified a novel breakpoint at 1p13 by 1Mb-spaced array CGH, and undertook a fine-tiling oligonucleotide array appro...
ORGANISM(S): Homo sapiens 
2007-03-21 | E-TABM-164 | biostudies-arrayexpress
TranscriptomicsIsocaloric intake of diets differing in macronutrient content (high protein or high fat) in adult rats indicate adverse effects as analysed by hepatic transcriptomics
Intake of high-protein (HP) diets has increased over the last years, mainly due to their popularity for body weight control. Liver is the main organ handling ingested macronutrients and it is associated with the beginning of different pathologies. We aimed to deepen our knowledge on molecular pathwa...
ORGANISM(S): Rattus norvegicus 
2017-05-02 | E-GEOD-57858 | biostudies-arrayexpress
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