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GenomicsGenome-wide erasure of DNA methylation in mouse primordial germ cells is affected by Aid deficiency
Epigenetic reprogramming including demethylation of DNA occurs in mammalian primordial germ cells (PGCs) and in early embryos, and is important for the erasure of imprints and epimutations, and the return to pluripotency. The extent of this reprogramming and its molecular mechanisms are poorly under...
ORGANISM(S): Mus musculus 
2010-01-26 | E-GEOD-19960 | biostudies-arrayexpress
GenomicsGenome-wide bisulfite sequencing in zygotes identifies demethylation targets and maps the contribution of TET3 oxidation
Fertilization triggers a global erasure of 5-methylcytosine from paternal DNA as part of extensive epigenetic reprogramming during the transition from gametic specialization to totipotency. This active removal has been shown to involve oxidation by TET3, but the targeting of this pathway and the wid...
ORGANISM(S): Mus musculus 
2014-12-22 | E-GEOD-63417 | biostudies-arrayexpress
GenomicsSingle-Cell Whole-Genome Bisulfite Sequencing for Analysis of Epigenetic Heterogeneity
We report a single-cell whole-genome bisulfite sequencing method (scBS-Seq) capable of accurately measuring DNA methylation at up to 36% of CpGs. We observed that ESCs grown in serum/LIF or 2i/LIF both display epigenetic heterogeneity, with M-bM-^@M-^\2i-likeM-bM-^@M-^] cells present in serum cultur...
ORGANISM(S): Mus musculus 
2014-07-18 | E-GEOD-56879 | biostudies-arrayexpress
GenomicsGenome-wide distribution of 5-formylcytosine in ES cells is associated with transcription and depends on TDG
Methylation of cytosine in DNA (5mC) is an important epigenetic mark that is involved in the regulation of genome function. During early embryonic development in mammals, the DNA methylation landscape is dynamically reprogrammed in part through active demethylation. Recent advances have identified k...
ORGANISM(S): Mus musculus 
2012-08-17 | E-GEOD-40148 | biostudies-arrayexpress
GenomicsPerturbed adult sperm methylome after in utero undernutrition is associated with transmission of metabolic disease
The pre and postnatal environment can affect both an individual’s risk of adult onset metabolic disease and that of subsequent generations. Although animal models and epidemiological data implicate epigenetic inheritance, little is known of the mechanisms involved. In a robust intergenerational mo...
ORGANISM(S): Mus musculus 
2014-07-16 | E-GEOD-58747 | biostudies-arrayexpress
UnknownA comprehensive approach for genome-wide efficiency profiling of DNA modifying enzymes.
Not available
S-EPMC9017147 | biostudies-literature
TranscriptomicsThe H19 lincRNA is a developmental reservoir of miR-675 which suppresses growth and Igf1r
Investigate the effect of an H19 knockout on gene expression
ORGANISM(S): Mus musculus 
2012-05-04 | E-MTAB-895 | biostudies-arrayexpress
GenomicsResetting Transcription Factor Control Circuitry Towards Ground State Pluripotency In Human
Current human pluripotent stem cells lack the transcription factor circuitry that governs the ground state of mouse embryonic stem cells (ESC). Here we report that short-term expression of two components, NANOG and KLF2, is sufficient to ignite other elements of the network and reset the human pluri...
ORGANISM(S): Homo sapiens 
2014-09-10 | E-GEOD-60945 | biostudies-arrayexpress
TranscriptomicsAffymetrix gene expression array (mouse 2.0) of sorted undifferentiated spermatogonia in inducible Miwi2 knock-out model
Undifferentiated spermatogonia were sorted from Miwi2Tom/FL; ERT-Cre/+ (Miwi2-iKO) and Miwi2Tom/+; ERT-Cre/+ (Miwi2-CTL) mice injected with tamoxifen two weeks after last tamoxifen injection to understand an impact of acute Miwi2 deletion on spermatogenic homeostasis.
ORGANISM(S): Mus musculus 
2018-03-21 | E-MTAB-6612 | biostudies-arrayexpress
TranscriptomicsKaryotype analysis of human naive embryonic stem cells
Human pluripotent cell lines were derived from blastocyst-stage embryos and propagated in self-renewal conditions that maintain features of naive pluripotency characteristic of mouse embryonic stem cells. Genomic integrity of the HNES1 cell line was assessed with the Affymetrix CytoScan 750K array.
ORGANISM(S): Homo sapiens 
2016-03-01 | E-MTAB-4463 | biostudies-arrayexpress
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