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ProteomicsInfluence of digestion protocol in Protein Quantitation
Quantitative determination of absolute and relative protein amounts is an essential requirement for most current bottom-up proteomics applications, but protein quantitation estimates are affected by several sources of variability such as sample preparation, mass spectrometric acquisition, and data a...
ORGANISM(S): Escherichia coli 
2014-07-30 | PXD001187 | Pride
Protein quantitation Digestion protocols Fasp 
ProteomicsRamie LC-MSMS - Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-Based Comparative Proteome Analysis of the Response of Ramie under Drought Stress
Ramie is an important natural fiber crop. Drought is an abiotic stress that strongly influences plant growth, development and productivity. There has been little research on the molecular mechanisms of ramie related to drought and then it is poorly understood. In this study, we conducted for the fir...
ORGANISM(S): Boehmeria nivea (Chinese grass) (Urtica nivea) 
2016-10-05 | PXD001940 | Pride
Plant proteomics Ramie Drought stress Itraq Seedlings 
ProteomicsQuantitation of ATIs in Wheat by LC-MS/MS – iTRAQ
A selection of ATIs was silenced in the common wheat cultivar Bobwhite and durum wheat cultivar Svevo by RNAi and gene editing, respectively, in order to reduce the amounts of ATIs. The controls and silenced lines were analyzed after digestion to peptides by LC-MS/MS with different approaches to eva...
ORGANISM(S): Triticum aestivum (Wheat) Triticum durum 
2022-10-13 | PXD034478 | Pride
Amylase/trypsin inhibitor  wheat  crsipr-cas9  rnai 
UnknownRElative QUantitation Inferred by Evaluating Mixtures (REQUIEM).
Not available
S-EPMC5735846 | biostudies-literature
ProteomicsHuman Plasma Proteome by Isobaric Tags for Relative and Absolute Quantitation Based Quantitative Proteomics Technique
This project was to identify relevant diagnostic biomarkers for vascular aging by performing a targeted proteomic comparison of plasma proteins between aortic stiffness (high PWV) and non-aortic stiffness (normal PWV) populations using isobaric tags for relative and absolute quantitation based qua...
ORGANISM(S): Homo Sapiens 
2021-12-19 | PXD030531 |
UnknownEvaluation of Spectral Counting for Relative Quantitation of Proteoforms in Top-Down Proteomics.
Not available
S-EPMC6178225 | biostudies-literature
ProteomicsSysQuan: repurposing SILAC mice for affordable absolute quantitation of the human proteome
Relative quantitation, used by most MS-based proteomics laboratories to determine protein fold-changes, requires that samples are processed and analyzed together to enable best comparability through minimizing batch differences. This limits the detection of subtle but relevant changes in heterogeneo...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-06-16 | PXD057266 | Pride
Proteomics Silac Stable isotope Targeted proteomics Absolute quantitation 
UnknownCharacterization of multiple myeloma vesicles by label-free relative quantitation.
Not available
S-EPMC3835174 | biostudies-literature
ProteomicsQuantitation of ATIs in Wheat by LC-MS/MS – LFQ Orbitrap
A selection of ATIs was silenced in the common wheat cultivar Bobwhite and durum wheat cultivar Svevo by RNAi and gene editing, respectively, in order to reduce the amounts of ATIs. The controls and silenced lines were analyzed after digestion to peptides by LC-MS/MS with different approaches to eva...
ORGANISM(S): Triticum aestivum (Wheat) Triticum durum 
2022-10-13 | PXD034458 | Pride
Amylase/trypsin inhibitor  wheat  crsipr-cas9  rnai 
UnknownImpact of peptide modifications on the isobaric tags for relative and absolute quantitation method accuracy.
Not available
S-EPMC3717298 | biostudies-literature
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