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ProteomicsA Metaproteomics Approach to Elucidate Host and Pathogen Protein Expression during Catheter-Associated Urinary Tract Infections
Long term-catheterization leads inevitably to a catheter-associated bacteriuria caused by multispecies bacterial biofilms growing on and in the catheters. The overall goal of the presented study was (I) to unravel bacterial community structure and function of such an uropathogenic biofilm and (II) t...
ORGANISM(S): Morganella <scale Insect> (ncbitaxon:108061) Morganella <fungus> (ncbitaxon:90690) Pseudomonas Sp. (ncbitaxon:306) Homo Sapiens (ncbitaxon:9606) 
2016-07-19 | MSV000079927 | MassIVE
ProteomicsProteome and carbon flux analysis of Pseudomonas aeruginosa clinical isolates from different infection sites
Pseudomonas aeruginosa is known as opportunistic pathogen frequently isolated from different infection sites such as burned wounds, lung and urinary tract. To shed light on the expression rates of cytoplasmic P. aeruginosa proteins, commonly expressed by eleven different clinical isolates, absolute ...
ORGANISM(S): Pseudomonas aeruginosa 
2016-03-29 | PXD002373 | Pride
Clinical isolates Gel-free and label-free proteomics 
ProteomicsAcidobacteria Granulicella sp. WH15 responses to carbon stress
Acidobacteria is a widely distributed phylum in soils, which possesses a relatively small number of cultured representatives. In general, Acidobacteria are considered to be oligothroph, able to succeed growth in poor nutrient environments and exhibiting slow growth under laboratory conditions. Howev...
ORGANISM(S): Granulicella sp. 
2020-05-27 | PXD015715 | Pride
Stress signal Proteome Transporters Genome Sigma factor σw Transcriptome Oligothroph 
ProteomicsCellulose and hemicellulose decomposition by forest soil bacteria proceeds by the action of structurally variable enzymatic systems
Evidence shows that bacteria contribute actively to the decomposition of cellulose and hemicellulose in forest soil; however, their role in this process is still unclear. Here we performed the screening and identification of bacteria showing potential cellulolytic activity from litter and organic so...
ORGANISM(S): Mucilaginibacter Pedobacter sp. Luteibacter 
2016-05-02 | PXD003844 | Pride
Decomposition Gelc-ms/ms Forest soil bacteria 
ProteomicsTerpenes a common language between fungi and bacteria
Previously, we investigated the effect of fungal VOCs on the behavior of phylogenetically different soil bacteria (Schmidt et al 2015). In these experiments we showed that VOCs emitted by several fungi can lead to phenotypical responses in bacteria, for example, by inducing a change in motility (Sch...
ORGANISM(S): Serratia plymuthica PRI-2C 
2018-07-19 | PXD004819 | Pride
Transcriptomics  proteomics  metabolomics  serratia plymuthica sp.  fusarium culmorum sp. Fungal-bacterial interactions  volatile organic compounds  terpenes 
ProteomicsDetailed sub-proteome analysis of a cheese-isolated Enterococcus faecalis, provide insights into probiotic value and food safety
Enterococci are normal inhabitants of the gastrointestinal tracts of humans and animals and thanks to their capability to tolerate different environmental conditions and their high rates of gene transfer, they are able to colonize various ecological niches, as food matrices. Enterococcus faecalis ba...
ORGANISM(S): Enterococcus faecalis str. Symbioflor 1 
2019-10-04 | PXD011660 | Pride
Virulence Mass spectrometry Host interaction Foodborne bacteria Antibiotic resistance 
ProteomicsVirulence factors produced by Staphylococcus aureus biofilms have a moonlighting function contributing to biofilm integrity
We comprehensively profiled intracellular and ECM proteomes of S. aureus flow biofilms and complemented these data by metabolic footprint analysis and phenotypic assays. We show that S. aureus biofilms produce high amounts of secreted, moonlighting virulence factors stabilizing the ECM. Mechanistica...
ORGANISM(S): Staphylococcus aureus subsp. aureus NCTC 8325 
2019-03-13 | PXD011157 | Pride
Ecm Biofilm Staphylococcus aureus 
ProteomicsDecoding the complete arsenal for cellulose and hemicellulose deconstruction in the highly efficient cellulose decomposer Paenibacillus O199
The search for new enzymes and microbial strains to degrade plant biomass is one of the most important strategies for improving the conversion processes in the production of environment-friendly chemicals and biofuels. In this study, we report a new Paenibacillus isolate, O199, which showed the high...
ORGANISM(S): Paenibacillus sp. 
2016-04-26 | PXD003970 | Pride
Proteomics Gelc-ms/ms Cellulose degradation 
ProteomicsA combined metaproteomics and metabolomics approach for the in vivo analysis of cystic fibrosis sputum focusing on microbial pathogens
Hallmarks of cystic fibrosis (CF) are increased viscosity of mucus and impaired mucociliary clearance within the airways due to mutations of the cystic fibrosis conductance regulator gene. This paves the way for the colonization by microbial pathogens and the concomitant establishment of chronic inf...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD025134 | Pride
Sputum Microbiome 16s sequencing Metabolomics In vivo Microbial community Cystic fibrosis Metaproteomics 
ProteomicsSystems-wide understanding of the osmotic response and metabolic rearrangement in Bacillus megaterium
For many years now, Bacillus megaterium has served as a microbial industrial strain for high-level production of recombinant proteins in the g/L-scale. Nevertheless, the impact of process-related stress has only been poorly characterized so far. Taking advantage of the recent technical developments ...
ORGANISM(S): Bacillus megaterium (strain DSM 319) 
2020-05-26 | PXD015605 | Pride
Proteomics Polyhydroxybutyrate Flux analysis Osmotic stress Proline Transcriptomics Bacillus megaterium 
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