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ProteomicsComprehensive evaluation of RNAlater effect on proteome and phosphoproteome
Tumors such as pancreatic cancer contain a variety of enzymes including DNases, RNases and proteases which may lead to autolysis of DNA, RNA and proteins during sample processing. In general, RNAlater can be used to keep nucleic acids intact since it contains high concentrations of quaternary ammoni...
ORGANISM(S): Homo sapiens (Human) 
2019-05-06 | PXD010710 | Pride
Rnalater Proteome Phosphoproteome 
TranscriptomicsTranscription profiling of fresh, frozen and RNALater preserved human uterine myometrium samples
We used quantitative RNA expression profiling on the Affymetrix U133 human expression array to validate quantitative expression results obtained with the tissue preservative RNALater against snap frozen and fresh tissues as a means of routine tissue collection and temporary storage. By using split ...
ORGANISM(S): Homo sapiens 
2007-06-21 | E-GEOD-1296 | biostudies-arrayexpress
ProteomicsComparative proteome stability analysis of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human colon mucosal biopsies
Clinical proteomics research is in part limited by the availability of clinical samples. However, large biobanks exist worldwide containing formalin-fixed and paraffin embedded samples and samples stored in RNAlater. The extraction of proteins for proteome analysis from samples prepared by either me...
ORGANISM(S): Homo sapiens (Human) 
2015-11-23 | PXD002029 | Pride
Human Snap-frozen Proteomics Mucosa Lc-ms Ffpe Rnalater Colon Stability 
TranscriptomicsTranscription profiling of human breast cancer or Dukes B colon cancer samples to compare the outcome from those fresh frozen and those stored in RNAlater preservative
A number of breast or colon specific genes predictive of the relapse status were used in comparing the outcome from matched fresh frozen and stored in RNAlater preservative. Experiment Overall Design: 31 pairs of matched breast and 21 pairs of matched colon primary tumors either fresh frozen or stor...
ORGANISM(S): Homo sapiens 
2007-10-02 | E-GEOD-3726 | biostudies-arrayexpress
ProteomicsComparative proteome stability analysis of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human colon mucosal biopsies
Clinical proteomics research is in part limited by the availability of clinical samples. However, large biobanks exist worldwide containing formalin-fixed and paraffin embedded samples and samples stored in RNAlater. The extraction of proteins for proteome analysis from samples prepared by either me...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-04-01 | MSV000080863 | MassIVE
UnknownNonrandom RNAseq gene expression associated with RNAlater and flash freezing storage methods.
Not available
S-EPMC6393184 | biostudies-literature
UnknownComprehensive proteome and phosphoproteome profiling shows negligible influence of RNAlater on protein abundance and phosphorylation.
Not available
S-EPMC6482574 | biostudies-literature
UnknownTranscriptome and proteome responses in RNAlater preserved tissue of Arabidopsis thaliana.
Not available
S-EPMC5397022 | biostudies-literature
TranscriptomicsStudy for evaluating the effect of cold ischemic time and RNA stabilization method on RNA integrity and gene expression measurements
Time series of eleven breast cancer samples subjected to different cold ischemic stress of up to 3 hr post tumor excision. A different 2x2 factorial within this study evaluated the effect of stabilization method (RNAlater vs snap freezing) and stablization delay (0 and 40 min) at room temperature. T...
ORGANISM(S): Homo sapiens 
2011-11-02 | E-GEOD-25011 | biostudies-arrayexpress
UnknownPrognostic gene expression signatures can be measured in tissues collected in RNAlater preservative.
Not available
S-EPMC1867564 | biostudies-literature
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