The RNA Polymerase II Associated Protein 1 (RPAP1) is conserved across metazoa and critical for stem cell differentiation in plants, however, very little is known about its mechanism of action or its role in mammalian cells. Here, we report that RPAP1 is essential for the expression of lineage spec...
Here, we investigated the role of RPAP1 in mammalian cell identity. As in plants, interfering with RPAP1 function did not affect the viability of mouse embryonic stem cells, but severely impaired their differentiation capacity (RNA-Seq#2 data, below). Conversely, in somatic cells, RPAP1 was essentia...
Effects of RPAP1 depletion on mRNA gene expression (RNA-seq) and RNA Pol II abundance (ChIP-seq) in primary mouse embryonic fibroblasts or embryonic stem cells.
