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ProteomicsIP-MS/MS analysis of UV induced RPII215 interactome
Drosophila Melanogaster has been extensively used as a model system to study ionizing radiation and chemical induced mutagenesis, double strand break repair and recombination. However, there are only limited studies on nucleotide excision repair in this important model organism. In this study, we im...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-04-04 | PXD028924 | Pride
Rpii215 Drosophila melanogaster Ip-ms/ms 
UnknownConservation of shibire and RpII215 temperature-sensitive lethal mutations between Drosophila and Bactrocera tryoni.
Not available
S-EPMC10926519 | biostudies-literature
TranscriptomicsTranscription elongation has a tissue-specific impact in alternative cleavage and polyadenylation in Drosophila melanogaster
Alternative cleavage and polyadenylation (APA) is a mechanism generating multiple mRNA isoforms with different 3'UTRs and/or coding sequences from a single gene. Here we used 3' Region Extraction And Deep Sequencing (3'READS) to systematically map cleavage and polyadenylation sites (PAS) in Drosophi...
ORGANISM(S): Drosophila melanogaster 
2017-08-23 | GSE85368 | GEO
TranscriptomicsNascent-Seq Indicates Widespread Cotranscriptional pre-mRNA Splicing in Drosophila
To determine the prevalence of cotranscriptional splicing in Drosophila, we sequenced nascent RNA transcripts from Drosophila S2 cells as well as from Drosophila heads. 87% of introns assayed manifest more than 50% cotranscriptional splicing. The remaining 13% are cotranscriptionally spliced poorly,...
ORGANISM(S): Drosophila melanogaster 
2011-12-01 | E-GEOD-32950 | biostudies-arrayexpress
GenomicsHog1 controls global reallocation of RNA Pol II upon osmotic shock
When challenged with osmotic shock, S. cerevisiae induces hundreds of genes, despite a global reduction in transcriptional capacity. The mechanisms that regulate this rapid reallocation of transcriptional resources are not known. Here we show that redistribution of RNA Pol II upon stress requires ...
ORGANISM(S): Saccharomyces cerevisiae 
2012-07-15 | E-GEOD-38208 | biostudies-arrayexpress
GenomicsGenome-wide mapping of RNA Pol-II promoter usage in mouse tissues by ChIP-seq
We report active promoters in five adult mouse tissues - brain, kidney, liver, lung and spleen using ChIP-Seq aganist RNAP-II antibody. We identified 38,639 active RNAP-II transcribed promoters, including 12,270 novel promoters. Of these, 6384 promoters are tissue specific which are CpG poor and con...
ORGANISM(S): Mus musculus 
2010-09-07 | E-GEOD-21773 | biostudies-arrayexpress
GenomicsPol II and its associated epigenetic marks are present at pol III-transcribed non-coding RNA genes (I)
Epigenetic control is an important aspect of gene regulation. Despite detailed understanding of many examples, the transcription of non-coding RNA genes by RNA polymerase (pol) III is less well characterized. Here we profile the epigenetic features of pol III target genes throughout the human gen...
ORGANISM(S): Homo sapiens 
2010-04-25 | E-GEOD-20040 | biostudies-arrayexpress
ProteomicsLC/MS Analysis of Myc-Affinity Captured, on-Beads Crosslinked, and on-Beads Digested S. cerevisiae SCC2 subunit of cohesin loading factor
Myc-Affinity Capture and on-Beads Crosslinking For each Saccharomyces cerevisiae strain (C-terminally myc-tagged Scc2 subunit of the cohesin loading factor complex or wild-type BY4741), 2x 3 L YPD medium (add 300 ml 20% Glucose to each 3 L of YPD before using) were separately inoculated in a 5L flas...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-10-20 | MSV000086339 | MassIVE
GenomicsRegulation of Mediator-dependent transcriptional activation of Pol II(G) through TFII
Pol II(G) is a novel form of RNA polymerase II that contains a tightly associated Gdown1 polypeptide. Whereas Pol II suffices for robust activator-dependent transcription in the absence of Mediator, Pol II(G) is highly dependent upon Mediator in a biochemically defined in vitro system. However, the ...
ORGANISM(S): Homo sapiens 
2012-01-13 | E-GEOD-33128 | biostudies-arrayexpress
TranscriptomicsChIP-chip by array of yeast JTY1, SHY546, BY4743, and Bur1 strains to investigate genome-wide patterns of RNA Polymerase II CTD Phosphorylation
Chromatin Immunoprecipitation in JTY1, SHY546, BY4743, and Bur1as strains using antibodies against RNA Pol II (RPB3) and its 3 phosphorylation states Ser2-P, Ser5-P, and Ser7-P, with and without inhibition with small molecule inhibitors.
ORGANISM(S): Saccharomyces cerevisiae 
2010-09-22 | E-MEXP-2850 | biostudies-arrayexpress
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