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ProteomicsRecombinant Protein Spectral Library (rPSL) DIA-MS Method Improves Identification and Quantification of Low-Abundance Cancer-Associated and Kynurenine Pathway Proteins
Data-independent acquisition mass spectrometry (DIA-MS) is a powerful tool for quantitative proteomics, but a well-constructed reference spectral library is crucial to optimize DIA analysis, particularly for low-abundance proteins. In this study, we evaluate the efficacy of a recombinant protein spe...
ORGANISM(S): Homo sapiens (Human) 
2025-05-15 | PXD061696 | Pride
Snap frozen tissues Rpsl Dia-ms Cancer- associated proteins Cell lysates Kynurenine pathway proteins Recombinant protein spectral library 
GenomicsEscherichia coli strain:NT1122
Restriction glycosylase mediated mutagenesis on rpsL in E.coli
PRJDB12250 | ENA
UnknownSensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection.
Not available
S-EPMC4349785 | biostudies-literature
UnknownProperties of Spontaneous rpsL Mutant of Streptomyces albus KO-1297.
Not available
S-EPMC8831875 | biostudies-literature
UnknownA suitable streptomycin-resistant mutant for constructing unmarked in-frame gene deletions using rpsL as a counter-selection marker.
Not available
S-EPMC4182516 | biostudies-literature
UnknownThe roles of rpsL, rrs, and gidB mutations in predicting streptomycin-resistant drugs used on clinical Mycobacterium tuberculosis isolates from Hebei Province, China.
Not available
S-EPMC6949554 | biostudies-literature
UnknownA novel mechanism of streptomycin resistance in Yersinia pestis: Mutation in the rpsL gene.
Not available
S-EPMC8096067 | biostudies-literature
UnknownUse of a dominant rpsL allele conferring streptomycin dependence for positive and negative selection in Thermus thermophilus.
Not available
S-EPMC1950985 | biostudies-literature
UnknownInteraction between rpsL and gyrA mutations affects the fitness and dual resistance of Mycobacterium tuberculosis clinical isolates against streptomycin and fluoroquinolones.
Not available
S-EPMC5877491 | biostudies-literature
UnknownA streptomycin resistance marker in H. parasuis based on site-directed mutations in rpsL gene to perform unmarked in-frame mutations and to verify natural transformation.
Not available
S-EPMC5767333 | biostudies-literature
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