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ProteomicsMetabolism as an early predictor of DPSCs aging
Tissue resident adult stem cells are known to participate in tissue regeneration and repair that follows cell turnover, or injury. It has been well established that aging impedes the regeneration capabilities at the cellular level, but it is not clear if the different onset of stem cell aging betwee...
ORGANISM(S): Homo sapiens (Human) 
2019-11-12 | PXD011962 | Pride
Aging stem cell 
ProteomicsAmino acid priming of mTOR is essential for heart regeneration
For a short period of time in mammalian neonates, the mammalian heart can regenerate via cardiomyocyte proliferation. This regenerative capacity is largely absent in adults. In other organisms, including zebrafish, damaged hearts can regenerate throughout their lifespans. Many studies have been perf...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2022-02-15 | PXD011791 | Pride
Heart regeneration Mtor 
ProteomicsFolliculin regulates mTORC1/2 and WNT pathways in early human pluripotency
Using co-immunoprecipitation and mass spectrometry analysis we identify unique FLCN binding partners. The interactions of FLCN with components of the mTOR pathway (mTORC1 and mTORC2) reveal a mechanism of FLCN function during exit from naïve pluripotency.
ORGANISM(S): Homo sapiens (Human) 
2019-02-13 | PXD011736 | Pride
Folliculin Mtor 
UnknownMetabolic remodeling in early development and cardiomyocyte maturation.
Aberrations in metabolism contribute to a large number of diseases, such as diabetes, obesity, cancer, and cardiovascular diseases, that have a substantial impact on the mortality rates and quality of life worldwide. However, the mechanisms leading to these changes in metabolic state--and whether th...
S-EPMC4820352 | biostudies-literature
UnknownMetabolism as an early predictor of DPSCs aging.
Tissue resident adult stem cells are known to participate in tissue regeneration and repair that follows cell turnover, or injury. It has been well established that aging impedes the regeneration capabilities at the cellular level, but it is not clear if the different onset of stem cell aging betwee...
S-EPMC6379364 | biostudies-literature
UnknownFirst critical repressive H3K27me3 marks in embryonic stem cells identified using designed protein inhibitor.
The polycomb repressive complex 2 (PRC2) histone methyltransferase plays a central role in epigenetic regulation in development and in cancer, and hence to interrogate its role in a specific developmental transition, methods are needed for disrupting function of the complex with high temporal and sp...
S-EPMC5617284 | biostudies-literature
UnknownProtocol for generating three-dimensional induced early ameloblasts using serum-free media and growth factors.
Adult humans cannot regenerate the enamel-forming cell type, ameloblasts. Hence, human induced pluripotent stem cell (hiPSC)-derived ameloblasts are valuable for investigating tooth development and regeneration. Here, we present a protocol for generating three-dimensional induced early ameloblasts (...
S-EPMC11176825 | biostudies-literature
UnknownFolliculin regulates mTORC1/2 and WNT pathways in early human pluripotency.
To reveal how cells exit human pluripotency, we designed a CRISPR-Cas9 screen exploiting the metabolic and epigenetic differences between naïve and primed pluripotent cells. We identify the tumor suppressor, Folliculin(FLCN) as a critical gene required for the exit from human pluripotency. Here we s...
S-EPMC6367455 | biostudies-literature
UnknownHypoxia-inducible factors have distinct and stage-specific roles during reprogramming of human cells to pluripotency.
Pluripotent stem cells have distinct metabolic requirements, and reprogramming cells to pluripotency requires a shift from oxidative to glycolytic metabolism. Here, we show that this shift occurs early during reprogramming of human cells and requires hypoxia-inducible factors (HIFs) in a stage-speci...
S-EPMC4028142 | biostudies-literature
UnknownLet-7 family of microRNA is required for maturation and adult-like metabolism in stem cell-derived cardiomyocytes.
In metazoans, transition from fetal to adult heart is accompanied by a switch in energy metabolism-glycolysis to fatty acid oxidation. The molecular factors regulating this metabolic switch remain largely unexplored. We first demonstrate that the molecular signatures in 1-year (y) matured human embr...
S-EPMC4450404 | biostudies-literature
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