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TranscriptomicsMiz-1 is required to coordinate the pre-TCR-dependent transitional step in DN3/DN4 thymocytes
Lineage negative, CD44 negative, CD25 positive thymocytes were isolated from wt mice or Miz1 POZ-domain knockout mice to analyze the effect of loss of Miz1 in the DN3 population of T-cells We used the mouse Affymetrix MOE430-2 microarray to characterize global gene expression changes of DN3 thymocy...
ORGANISM(S): Mus musculus 
2011-08-23 | E-GEOD-28342 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of naive CD4+ and CD8+ T-cells from Gfi1-knockout and wild type mice
to compare the transcriptomes of naïve CD4+ and CD8+ T-cells from Gfi1-knockout cells with wildtype cells, spleenocytes from wildtype and Gfi-knockout C57Bl/6 mice were isolated, stained with CD44, CD4 and CD8 antibodies and separated by using a FACS Diva (Becton Dickinson). Total RNAs isolated fr...
ORGANISM(S): Mus musculus 
2008-10-17 | E-GEOD-4938 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse T-cells from wildtype and Gfi1 knockout mice
This SuperSeries is composed of the following subset Series:; GSE4938: murine CD4+ and CD8+ T-cells from wildtype and Gfi knockout cells; GSE4940: murine T-cells from wildtype and Gfi1 knockout mice activated by anti-CD3 plus anti-CD28 for 0 to 24h Experiment Overall Design: Refer to individual Seri...
ORGANISM(S): Mus musculus 
2009-08-07 | E-GEOD-4950 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse T-cells from wildtype and Gfi1 knockout mice activated by anti-CD3 plus anti-CD28 for 0 to 24h
To compare the transcriptomes of T-cells from Gfi1-knockout cells with wildtype cells, spleenocytes from wildtype and Gfi-knockout C57Bl/6 mice were isolated using panT-cell kit in an AutoMACS device (Miltenyi). Cells were cultured with anti-CD3 plus anti-CD28 antibodies (2 µg antibody/ml) for th...
ORGANISM(S): Mus musculus 
2008-10-18 | E-GEOD-4940 | biostudies-arrayexpress
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