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ProteomicsA comparison of blood plasma exosome enrichment strategies for proteomic analysis.
Proteomic analysis of exosomes (EXs) poses a significant challenge. A ‘gold-standard’ method for plasma EX enrichment for downstream proteomic analysis is yet to be established. Methods were evaluated for their capacity to successfully isolate and enrich EXs from plasma, minimise the presence of hig...
ORGANISM(S): Bos taurus (Bovine) 
2022-08-12 | PXD032313 | Pride
Exosome  isolation  enrichment  ultracentrifugation  size-exclusion chromatography  ultrafiltration  mass-spectrometry  proteomics  extracellular vesicles 
ProteomicsAutomated proteomics workflows for consistent library generation and label-free protein quantitation in 96-well format and across different samples types
Automated workflows for high-throughput library generation and biomarker detection using data-independent acquisition
ORGANISM(S): Bos taurus (Bovine) 
2023-04-22 | PXD038413 | Pride
Myocardial infacrction Cattle Sheep 
ProteomicsPROTOTYPE OF THE CIRCULATING ACELLULAR PROTEOME OF HEALTHY SHEEP USING SERUM
This project deals with the characterisation and establishment of the baseline circulating acellular proteome of sheep serum using generic methods. Serum is a readily available substrate for most experimental and clinical applications as evidenced in recent sheep studies. It involves sample preparat...
ORGANISM(S): Ovis aries 
2017-06-19 | PXD004989 | Pride
Nanolc-nanoesi-ms/ms Serum Circulating acellular proteome Sheep 
ProteomicsAPPLICATION OF A NOVEL PEPTIDE SPECTRAL LIBRARY USING SWATH ANALYSIS FOR THE QUANTITATION OF PROTEINS IN EX-DIAGNOSTIC CLINICAL SERUM SAMPLES FROM SHEEP
This project applies SWATH approach to analyse ex-diagnostic sheep serum samples from actual clinical cases submitted to The University of Queensland's School of Veterinary Science, Veterinary Laboratory Services (VLS) compared with serum of normal sheep.
ORGANISM(S): Ovis aries 
2022-11-15 | PXD005077 | Pride
Nanolc-nanoesi-ms/ms Data independent acquisition Sick sheep Swath Peptide spectral library Proteogenomics Circulating acellular proteome Tripletof 5600+ Sample preparation 
ProteomicsTowards a comprehensive targeted proteogenomic assay repository for the liquid fraction of sheep blood
This dataset presents the results of protein sample preparation strategies for enriching a novel encyclopaedic peptide spectral library using the liquid fraction of sheep blood. The method used showed the capability of simultaneously detecting hundreds of key proteins in the liquid fraction of blood...
ORGANISM(S): Ovis aries 
2022-11-15 | PXD005002 | Pride
Nanolc-nanoesi-ms/ms Peptide spectral library Sheep Proteogenomics Circulating acellular proteome Data dependent acquisition Tripletof 5600+ 
ProteomicsDesign, Implementation and Multisite Evaluation of a System Suitability Protocol for the Quantitative Assessment of Instrument Performance in Liquid Chromatography-Multiple Reaction Monitoring-MS (LC-MRM-MS)
Multiple reaction monitoring (MRM) mass spectrometry coupled with stable isotope dilution (SID) and liquid chromatography (LC) is increasingly used in biological and clinical studies for precise and reproducible quantification of peptides and proteins in complex sample matrices. Robust LC-SID-MRM-MS...
ORGANISM(S): Bos Taurus 
PXD010535 | panorama
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