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Proteomics2D-LC/MS/MS analysis of Escherichia coli during exposure to abrupt downshifts in temperature and water activity
2D-LC/MS/MS analysis was used to examine time-dependent changes in proteome of E. coli O157:H7 strain Sakai upon abrupt downshifts from 35°C aw 0.993 to 14°C aw 0.967. Bacterial cells were harvested before abrupt downshifts in both temperature and aw (i.e. control), or at 0 (i.e. immediately after t...
ORGANISM(S): Escherichia coli 
2016-09-29 | PXD004803 | Pride
Cold temperature 2d-lc/ms/ms Water activity stress E. coli 
GenomicsEscherichia coli O157:H7 str. Sakai
Escherichia coli O157:H7 str. Sakai Genome sequencing
PRJNA171729 | ENA
Proteomics2D-LC/MS/MS analysis of Escherichia coli under optimal growth condition
2D-LC/MS/MS analysis was used to examine the proteome of E. coli O157:H7 strain Sakai during exponential growth under optimal condition (i.e., from 35°C aw 0.993). MS/MS data obtained from each protein sample were processed by the Computational Proteomics Analysis System (CPAS), a web-based system ...
ORGANISM(S): Escherichia coli 
2019-12-04 | PXD000583 | Pride
Optimal growth condition Physiology 2d-lc/ms/ms E. coli 
TranscriptomicsTranscriptome of Escherichia coli O157:H7 (Sakai) in response to spinach leaf lysates by RNA-seq
E. coli O157:H7 can use vegetables such as spinach as secondary hosts, from where they can be transmitted into the food chain. Our previous microarray analysis showed whole-scale changes in gene expression of E. coli driven in a large part in response to plant metabolites. The aim of this work was t...
ORGANISM(S): Escherichia coli 
2018-12-13 | E-MTAB-7441 | biostudies-arrayexpress
TranscriptomicsThe RpoN Regulon of Escherichia coli O157:H7
There is increasing evidence to support a role for sigma factor 54 (RpoN) in the regulation of stress resistance factors and protein secretion systems important to bacterial transmission and pathogenesis. In enterohemorrhagic E. coli O157:H7, acid resistance and type III secretion are essential det...
ORGANISM(S): Escherichia coli O157:H7 str. Sakai 
2009-08-08 | E-GEOD-17467 | biostudies-arrayexpress
TranscriptomicsMolecular response of Escherichia coli O157:H7 Sakai during dynamic changes in growth kinetics induced by an abrupt downshift in temperature and water activity
This experiment contains the transcriptomic dataset that constitutes part of an integrated transcriptomic and proteomic study monitoring the response of exponential phase E. coli O157:H7 Sakai cultures upon an abrupt downshift in temperature and water activity (from 35°C aw 0.993 to 14°C aw 0.967)...
ORGANISM(S): Escherichia coli 
2016-09-16 | E-MTAB-3969 | biostudies-arrayexpress
TranscriptomicsThe plasmid-borne Type II Secretion System of Escherichia coli O157:H7 isolate Sakai mediates adherence to spinach. Experiment 1: screening E coli O157:H7 Sakai genes for adherence to spinach roots.
Food-borne illness arising for Shiga-toxigenic Escherichia coli is often linked to consumption of fruit and vegetables as the bacteria have the ability to interact with plants and use them as alternative or secondary hosts. Attachment of the bacteria to host tissue is one of the first steps in the ...
ORGANISM(S): Escherichia coli 
2019-11-20 | E-MTAB-5923 | biostudies-arrayexpress
GenomicsRNAseq of E. coli Sakai O157:H7 in the presence of the flexicate compound Λ-5b
RNAseq of E. coli Sakai O157:H7 in the presence of the flexicate compound Λ-5b
PRJNA516945 | ENA
GenomicsEscherichia coli strain:O157:H7 Sakai (EHEC)
Escherichia coli strain:O157:H7 Sakai (EHEC) Raw sequence reads
PRJNA590589 | ENA
TranscriptomicsTranscriptional profiling of E. coli K-12 and E. coli O157 (Sakai) following heat shock at 45 degree celsius
Escherichia coli O157 presents a number of specific problems in terms of food safety and public health. It has been found that E. coli O157 is more resistant to a number of the stresses encountered during food production such as heat, pH and osmotic shock. This greater resistance is thought to contr...
ORGANISM(S): Escherichia coli 
2018-03-20 | E-MTAB-5912 | biostudies-arrayexpress
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