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ProteomicsLabel-free quantitative proteomics of enriched nuclei from sugarcane (Saccharum ssp) stems in response to drought stress
Drought is considered the major abiotic stress limiting crop productivity. This study sought to identify proteins involved in the drought response in sugarcane stems submitted to drought stress. The integration of nuclei enrichment sample preparation with the shotgun proteomic approach (nLC-MS/MS) r...
ORGANISM(S): Saccharum sp. 
2019-06-10 | PXD013797 | Pride
Rna regulation Shotgun proteomics Drought Transcription factor. Saccharum spp 
ProteomicsProteomics of phage VPE25 treated Enterococcus faecalis OG1RF relative to untreated controls
E. faecalis OG1RF cultures were infected with lytic phage VPE25 and proteomic profile of infected cells were compared to uninfected E. faecalis cells using 1D-LC-MS/MS analyses.
ORGANISM(S): Enterococcus phage VPE25 Enterococcus faecalis OG1RF 
2024-07-27 | PXD026873 | Pride
Enteroccocus faecalis Proteomics Phage vpe25 1d-lc-ms/ms 
ProteomicsEnvironmental viscosity modulates interbacterial killing during habitat transition
In this study we employed a proteomics method to determine how bacteria respond at the protein level to a transition from a low- to a high-viscosity liquid environment. We applied this method to explore how the symbiotic marine bacterium Vibrio fischeri responds to changes in environmental viscosity...
ORGANISM(S): Aliivibrio fischeri 
2020-01-07 | PXD015403 | Pride
Aliivibrio fischeri Squid-vibrio symbiosis Vibrio fischeri Symbiont 1d-lc-ms/ms Type vi secretion system 
ProteomicsOptimization of protein extraction methods for root-associated microbes in Arabidopsis
The goal of this study was to optimize protein extraction methods to study root-associated bacteria in Arabidopsis. For this we inoculated Arabidopsis seedlings grown in agar plates with a synthetic community (SynSom) composed of four different strains (Variovorax paradoxus, Arthrobacter sp, Agrobac...
ORGANISM(S): Pseudomonas Arabidopsis thaliana (Mouse-ear cress) Agrobacterium Arthrobacter Variovorax paradoxus 
2022-09-08 | PXD026330 | Pride
Protein extraction Metaproteome Roots Microbiome Arabidopsis Plant microbiota Plant-microbe interactions Metaproteomics 
ProteomicsOptimization of protein extraction methods for root-associated microbes in maize
The goal of this study was to optimize protein extraction methods to study root-associated bacteria in maize. For this we inoculated sterile maize plants with a synthetic community composed of seven different bacteria (Ben Niu et al. PNAS 2017, vol 114, n 12). Then, we extracted proteins from maize ...
ORGANISM(S): Herbaspirillum robiniae Ochrobactrum pituitosum Enterobacter ludwigii Zea mays (Maize) Curtobacterium pusillum Pseudomonas putida Chryseobacterium indologenes 
2022-09-08 | PXD026369 | Pride
Protein extraction Metaproteome Roots Microbiome Corn Maize Plant microbiota Plant-microbe interactions Metaproteomics 
ProteomicsA strain-specific genomic island encodes regulatory proteins to activate type VI secretion system expression in the squid symbiont Vibrio fischeri.
We used the bioluminescent squid symbiont, Vibrio fischeri, to identify essential regulatory factors that control expression and function of a strain-specific T6SS encoded within a genomic island. Random transposon mutagenesis revealed that three genes located on the T6SS2-encoding genomic island ar...
ORGANISM(S): Aliivibrio fischeri 
2021-08-24 | PXD017722 | Pride
Aliivibrio fischeri Squid-vibrio symbiosis Genomic island Symbiosis Vibrio fischeri 1d-lc-ms/ms Type vi secretion system 
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