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ProteomicsDevelopment of an Adaptive, Economical, and Easy-to-Use SP3-TMT Automated Sample Preparation Workflow for Quantitative Proteomics
Liquid handling robots have been developed to automate various steps of the bottom-up proteomics workflow, however, protocols for the generation of isobarically labeled peptides remain limited. Existing methods often require costly specialty devices and are constrained by fixed workflows. To address...
ORGANISM(S): Homo sapiens (Human) 
2025-06-16 | PXD060786 | Pride
Automation Sample preperation Unfolded protein response and tandem mass tags 
ProteomicsHeat ‘n Beat: A universal high-throughput end-to-end proteomics sample processing platform in under an hour
Clinical proteomics on solid tissue samples from human tumour biopsies present multiple challenges. These include limited sample availability, reducing endogenous protease activity and challenges with efficiently solubilising and digesting proteins for high throughput mass spectrometry while achievi...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2025-03-26 | PXD045405 | Pride
Sample preperation Proteomics High-throughput Heat n beat Universal Mass spec 
ProteomicsnPOP_ProtocolPaper_Dataset_celllines
WM989, u937, and CPAF cell lines run on the timsTOF Ultra. Purpose is to benchmark and showcase nPOP sample preperation.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-11-25 | MSV000093494 | MassIVE
TranscriptomicsRNA seq of different species of Salsoleae with different photosynthetic types
Cotyledons and leaf transcriptomes of species of Salsoleae with different photosynthetic types were de novo assembled and analyzed to provide a better understanding of differential gene expression between C3, C2 and C4 species. Total RNA of cotyledons and leaves of different species of Salsoleae wi...
ORGANISM(S): Salsola webbii 
2017-12-29 | E-MTAB-5778 | biostudies-arrayexpress
TranscriptomicsRNA sequencing of Control, Î?Cop1β, Î?Cop1β and Î?Cop1β Î?ETV1/4/5β islets
Identify genes which are differentially expressed in Î?Cop1β compared to control islets and are also signficantly rescued in Î?Cop1Î? Î?ETV1/4/5β Islets were harvested from 5 biological replicates of the followig genotypes: Control, Î?Cop1β and Î?Cop1Î? Î?ETV1/4/5β. Islets were individually han...
ORGANISM(S): Mus musculus 
2015-11-09 | E-GEOD-70788 | biostudies-arrayexpress
TranscriptomicsIdentification of differentially expressed genes in fore and hind wing buds of IV instar larvae of Bombyx mori (Daizo)
Amongst the various different insect groups, there is remarkable diversity in the number and size of wings. However the development of the basic body plan in insects is similar to a large extent. The genes of the hox complex regulate various pathways to bring about the development or modification of...
ORGANISM(S): Bombyx mori 
2016-07-27 | E-GEOD-71988 | biostudies-arrayexpress
GenomicsChIPseq iEC reprogramming
ChIPseq iEC reprogramming
PRJNA1116102 | ENA
GenomicsH3K27ac ChIPseq of the ecDNA-positive colon cancer cell line COLO 320DM with and without hydroxyurea treatment
Extrachromosomal DNA (ecDNA) is a major driver of oncogene amplification, intratumoural heterogeneity and rapid genetic change. We observe that ecDNA frequently undergoes segregation errors and is incorporated into micronuclei (MN) in ecDNA positive cancer cells. Different ecDNA species can coalesce...
ORGANISM(S): Homo sapiens 
2026-07-02 | E-MTAB-16944 | biostudies-arrayexpress
TranscriptomicsNeural Stem Cells Direct Axon Guidance via their Radial Fiber Scaffold
To determine the genes regulated by Fezf2 during neocortical development, we performed RNA-seq on Fezf2 +/+; Emx1-cre (Fezf2 wildtype) and Fezf2 fl/+; Emx1-Cre (Fezf2 Knockout) neocortices isolated from the P0 mice. After RNA isolation from the dissected tissue the samples were processed for library...
ORGANISM(S): Mus musculus 
2020-12-05 | GSE152611 | GEO
UnknownBlood collection in cell-stabilizing tubes does not impact germline DNA quality for pediatric patients.
Not available
S-EPMC5716571 | biostudies-literature
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