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ProteomicsSample preparation for proteomics
Urea-containing buffer solutions are generally used in proteomic studies to aid protein denaturation and solubilisation during cell and tissue lysis. It is well-known, however, that urea can lead to the carbamylation of peptides and proteins and; subsequently, incomplete digestion of proteins. In...
ORGANISM(S): Rattus rattus (Black rat) Homo sapiens (Human) 
2018-06-06 | PXD009426 | Pride
Lys-c/trypsin Urea Lc-ms/ms 
ProteomicsGlobal identification of biofilm-specific proteolysis in Candida albicans
The global extracellular protease substrate specificity of C. albicans under biofilm and planktonic (suspension) conditions was determined with a synthetic library of 14-mer peptide substrates using an approach termed Multiplex Substrate Profiling by Mass Spectrometry (MSP-MS). Shotgun proteomics an...
ORGANISM(S): Candida Albicans (ncbitaxon:5476) 
2016-03-09 | MSV000079564 | MassIVE
UnknownSame data, different analysts: variation in effect sizes due to analytical decisions in ecology and evolutionary biology.
Not available
S-EPMC11804095 | biostudies-literature
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