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ProteomicsExtending the coverage of Lys-C/trypsin-based bottom-up proteomics using cysteine S-aminoethylation
In this study, S-aminoethylation of Cys (AE-Cys) was carried out with 2-bromoethylamine to form pseudo-Lys, which was then digested with Lys-C or trypsin to improve proteome coverage in bottom-up proteomics. A model study with bovine serum albumin showed that the C-terminal side of Cys was successfu...
ORGANISM(S): Homo Sapiens (human) Bos Taurus 
2023-09-27 | PXD045433 | JPOST Repository
ProteomicsLys-C/Arg-C, a New Enzyme Combination for Next-Generation Proteomics
Today, the most widely used bottom-up proteomics studies necessitate a proteolysis step prior to MS analysis. Trypsin is often the best protease in choice due to its high specificity and MS-favored proteolytic products. Trypsin has been challenged for its low cleavage efficiency at Lys-X bonds, and ...
ORGANISM(S): Homo sapiens (Human) 
2018-07-24 | PXD009797 | Pride
Lys-c / arg-c / trypsin / dia / mass spectrometry 
ProteomicsReversible Lysine Derivatization Enables High Specificity of Arg-C Digestion Using Trypsin
Bottom-up proteomics approach has become an important strategy in diverse areas of biological research, and the enzymatic digestion is essential for this technology. Endopeptidase Arg-C catalyzing the hydrolytic cleavage of peptide bonds C-terminal to arginine could be an important protease in botto...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-01-04 | PXD007994 | Pride
Proteomics Arg-c Trypsin Lys-c Citraconylation Lc-ms/ms 
ProteomicsSample preparation for proteomics
Urea-containing buffer solutions are generally used in proteomic studies to aid protein denaturation and solubilisation during cell and tissue lysis. It is well-known, however, that urea can lead to the carbamylation of peptides and proteins and; subsequently, incomplete digestion of proteins. In...
ORGANISM(S): Rattus rattus (Black rat) Homo sapiens (Human) 
2018-06-06 | PXD009426 | Pride
Lys-c/trypsin Urea Lc-ms/ms 
ProteomicsIdentification of zein proteome by multi-enzyme digestion
Zeins are a class of prolamine proteins extracted from maize, and are extensively used in the food and pharmaceutical industries. Therefore, characterization of its components is essential for quality control and safety evaluation. However, zein proteins have low abundance of the trypsin targets lys...
ORGANISM(S): Zea Mays 
2019-07-25 | PXD014743 |
ProteomicsDDA-Lys-C/Trypsin-C4 SPE
Not available
2021-04-20 | MSV000076896 | MassIVE
ProteomicsLipopolysaccharide-induced changes in the RNA-binding proteome of mouse macrophages
SILAC-labeled immortalized mouse macrophages (IMMs) were LPS-stimulated. RNA-associated proteins were crosslinked to RNA using UV. RNA crosslinked proteins were purified, trypsin + Lys-C digested, and analyzed using LC-MS proteomics.
ORGANISM(S): Mus musculus (Mouse) 
2024-02-27 | PXD046754 | Pride
Macrophage Lps Mouse Lc-ms Rna-binding protein Proteomics 
ProteomicsShake and bake: a robust and cost-effective sample preparation recipe for plasma and cerebrospinal fluid
Background Plasma and cerebrospinal fluid CSF are complementary sources of biomarkers for neurodegenerative diseases. However, the wide dynamic range of protein abundances, particularly in plasma, hinders detection of low-abundance proteins. Depletion of high-abundance proteins and efficient enzymat...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-10-29 | MSV000099682 | MassIVE
ProteomicsDiNovo enhances de novo peptide sequencing coverage and confidence via mirror proteases and deep learning
this study employs two pairs of mirror ptoteases, trypsin/LysargiNase,and Lys-C/Lys-N to digest E.coli or yeast proteomes to evaluate a new software for de novo sequecing.
ORGANISM(S): Homo Sapiens Escherichia Coli Saccharomyces Cerevisiae 
2026-01-31 | PXD059331 |
ProteomicsLC-MS peptide mapping of monoclonal antibodies using the mirror proteases trypsin and Tryp-N
Accurate characterization of the amino acid sequence and post-translational modifications (PTMs) of monoclonal antibodies (mAbs) is essential for evaluating product quality. Peptide mapping through bottom-up LC/MS analysis is a key methodology for this purpose. While trypsin is commonly the first ch...
ORGANISM(S): Homo Sapiens (human) 
2026-04-15 | PXD072803 | JPOST Repository
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