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Proteomics‘Off the shelf’ proximity biotinylation for interaction proteomics
Proximity biotinylation workflows typically require CRISPR-based genetic manipulation of target cells. To overcome this bottleneck, we fused the TurboID proximity biotinylation enzyme to Protein A. Upon target cell permeabilization, the ProtA-Turbo enzyme can be targeted to proteins of interest usin...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-07-12 | PXD025012 | Pride
Flywch1 Proximity labelling Turboid Interactome Protein a 
ProteomicsOff the shelf proximity biotinylation using ProtA-TurboID
Proximity biotinylation is a commonly used method to identify the in vivo proximal proteome for proteins of interest. This technology typically relies on fusing a bait protein to a biotin ligase using overexpression or CRISPR-based tagging, thus prohibiting such assays in (primary) cell types that a...
ORGANISM(S): Homo sapiens (Human) 
2022-10-17 | PXD030963 | Pride
Cell lines Centromeres Biotinylation Histone ptms Protein-protein interactions 
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