Sort   by:  
 Page size 
ProteomicsLFQ of biotinylated peptides from HEK293T cells expressing AirID-CRBN
HEK293T cells stably expressing AGIA-AirID-CRBN-WT were treated with DMSO, 20 µM thalidomide, 10 µM lenalidomide, 10 µM pomalidomide, or 20 µM 5-hydroxythalidomide (5HT) in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. After tryptic digestion, biotinylated pepti...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028756 | JPOST Repository
ProteomicsPIM kinases facilitate lentiviral evasion from SAMHD1 restriction via Vpx phosphorylation
Proteomic analysis revealed potential phospho-acceptor sites within Vpx by PIM3. HEK293 cells were transfected with plasmid vector encoding Vpx together with either empty vector or PIM3. Immunoprecipitated Vpx was subjected to liquid chromatography tandem-mass spectrometry.
ORGANISM(S): Homo Sapiens (human) 
2019-03-20 | PXD013154 | JPOST Repository
ProteomicsThBD-AGIA-AirID expressing MM1.S cells treated with DMSO, Pomalidomide, GA-TD-3 or GA-TD-5
MM1.S cells stably expressing ThBD-AGIA-AirID were treated with DMSO, Pomalidomide, GA-TD-3 or GA-TD-5 with MG132 in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and ide...
ORGANISM(S): Homo Sapiens (human) 
2025-09-03 | PXD067146 | JPOST Repository
ProteomicsThBD-AGIA-AirID expressing HEK293T cells treated with DMSO, Pomalidomide, GA-TD-3 or GA-TD-5
HEK293T cells stably expressing ThBD-AGIA-AirID were treated with DMSO, Pomalidomide, GA-TD-3 or GA-TD-5 with MG132 in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and i...
ORGANISM(S): Homo Sapiens (human) 
2025-09-03 | PXD067145 | JPOST Repository
ProteomicsVHL-AirID expressing HCC827-Luc cells treated with DMSO, VH032 or MS39
HCC827-Luc cells stably expressing VHL-AirID were pretreated with MG132. The DMSO, VH032 or MS39 were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV ...
ORGANISM(S): Homo Sapiens (human) 
2025-09-03 | PXD067144 | JPOST Repository
ProteomicsThBD-AGIA-AirID expressing HCC827-Luc cells treated with DMSO, Pomalidomide or MS154
HCC827-Luc cells stably expressing ThBD-AGIA-AirID were pretreated with MG132. The DMSO, Pomalidomide or MS154 were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Ta...
ORGANISM(S): Homo Sapiens (human) 
2025-09-03 | PXD067143 | JPOST Repository
ProteomicsThBD-AGIA-AirID expressing MM1.S cells treated with DMSO, Pomalidomide or ARV-825
MM1.S cells stably expressing ThBD-AGIA-AirID were treated with DMSO, Pomalidomide or ARV-825 with MG132 in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified b...
ORGANISM(S): Homo Sapiens (human) 
2025-09-03 | PXD067142 | JPOST Repository
ProteomicsVHL-AirID expressing MM1.S cells treated with DMSO, VH032 or MZ1
MM1.S cells stably expressing VHL-AirID were treated with DMSO, VH032 or MZ1 with MG132 in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS analys...
ORGANISM(S): Homo Sapiens (human) 
2025-09-03 | PXD067141 | JPOST Repository
ProteomicsThBD-AGIA-AirID expressing LNCaP cells treated with Pomalidomide or ARM-110
LNCaP cells stably expressing ThBD-AGIA-AirID were pretreated with MG132. Pomalidomide or ARV-110 was added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV ...
ORGANISM(S): Homo Sapiens (human) 
2025-09-03 | PXD067147 | JPOST Repository
ProteomicsComparison of three enrichment methods of biotinylated peptides or proteins from AirID-CRBN-expressing MM1.S cells
MM1.S cells stably expressing AGIA-AirID-CRBN were treated with DMSO or 10 µM pomalidomide in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. The cells were divided into three tubes and lysed. After tryptic digestion, biotinylated peptides were enriched using Tama...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028754 | JPOST Repository
Sort   by:  
 Page size