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ProteomicsSynthetic polyclonal-derived CDR peptides as innovative strategy in glaucoma therapy
The pathogenesis of glaucoma is strongly associated with the occurrence of autoimmune-mediated loss of retinal ganglion cells (RGCs) and recently it was proven that specific antibody-derived signature peptides are significantly differentially expressed in sera of primary open angle glaucoma patients...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2019-08-15 | PXD014718 | Pride
Glaucoma  autoimmunity  synthetic cdr peptides  neuroprotection  htra2  sus scrofa domesticus 
ProteomicsComparison of two solid-phase extraction (SPE) methods for the identification and quantification of porcine retinal protein markers by LC MS/MS
Proper sample preparation protocols represent a critical step for LC-MS-based proteomic study designs and influence the speed, performance and automation of high-throughput data acquisition. The main objective of this study was to compare two commercial solid phase extraction (SPE)-based sample prep...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2018-12-03 | PXD011755 | Pride
Mass spectrometry  glaucoma animal model  biomarkers  sample clean-up  ziptip® c18 pipette tips  solaμtm hrp spe spin plates 
ProteomicsRegulation of the HTRA2 protease activity by PDZ domain-binding peptide ligands and the influence on HTRA2-specific protein interaction networks in retinal tissues
For the identification of HTRA2-specific protein interaction partners in retinal tissues of the house swine (Sus scrofa), we performed co-immunoprecipitation experiments with recombinant 6His-tag HTRA2 followed by quantitative LC-MS-based proteomics (see Fig. 2A and File S1). In brief, 2 µg recombin...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2021-10-11 | PXD024442 | Pride
Htra2  neuroprotection  retina  interaction partners  co-immunoprecipitation  mass spectrometry 
ProteomicsLectin-based affinity enrichment and characterization of N-glycoproteins from the human tear film by mass spectrometry
Glycosylation of proteins is one of the most common post-translational modification (PTM) and plays important regulatory functions in diverse biological processes such as protein stability or cell signaling. Accordingly, glycoproteins are also a consistent part of the human tear film proteome mainta...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2023-01-25 | PXD038280 | Pride
N-glycoproteins Mass spectrometry Pngase f Tear film Multi-lectin column 
ProteomicsSynthetic antibody-derived immunopeptide provides neuroprotection in glaucoma through molecular interaction with retinal protein Histone H3.1
Glaucoma is a group of optic neuropathies characterized by the progressive degeneration of retinal ganglion cells (RGCs) as well as their axons leading to irreversible loss of sight. Medical management of the intraocular pressure (IOP) still represents the gold standard in glaucoma therapy, which on...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2022-11-09 | PXD034046 | Pride
Glaucoma Cdr2 Neuroprotection Hist1h3a 
ProteomicsGlaucoma-Associated CDR1 Peptide Promotes RGC Survival in Retinal Explants through Molecular Interaction with Acidic Leucine Rich Nuclear Phosphoprotein 32A (ANP32A)
Glaucoma is a complex, multifactorial optic neuropathy mainly characterized by the progressive loss of retinal ganglion cells (RGCs) and their axons, resulting in a decline of visual function. The pathogenic molecular mechanism of glaucoma is still not well understood, and therapeutic strategies spe...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2023-08-01 | PXD038373 | Pride
Glaucoma Neuroprotection Mass spectrometry Proteomics Anp32a 
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