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TranscriptomicsChromatin immunoprecipitation of Drosophila Schneider cells using antibodies against HP1a
Preparation of nuclear extracts

D. melanogaster Schneider cells were prepared as previously described by Andrews and Faller (1991). Briefly, in a typical preparation, 8x106 cells (grown in Schneider medium plus 10% fetal calf serum; GIBCO-BRL) were pelleted and resuspended in 1,5 mL cold PBS1...
ORGANISM(S): Drosophila melanogaster 
2009-10-12 | E-MEXP-1556 | biostudies-arrayexpress
TranscriptomicsTiling path analysis of Drosophila Schneider 2 cultured cells to map forum domains
Forum domains are stretches of chromosomal DNA that are excised from eukaryotic chromosomes during their spontaneous non-random fragmentation. Mostly forum domains are of 50-200 kb in length, although larger domains, up to 500 - 700 kb, are also observed.

We performed a genome-wide mapping o...
ORGANISM(S): Drosophila melanogaster 
2010-11-01 | E-MEXP-2468 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of CACNA1E overexpression in human embryonic kidney HEK293 cells to investigate the involvement of CACNA1E in Wilms tumorigenesis
Wilms tumour (nephroblastoma) is the most common paediatric renal tumour, affecting 1 in 10,000 children. Despite the excellent survival of patients treated with a combination of surgery, chemotherapy and radiotherapy, approximately 15% will relapse and die of this disease. We have identified CACNA1...
ORGANISM(S): Homo sapiens 
2006-12-01 | E-MEXP-587 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of Drosophila Schneider 2 cells using NELF-E and NELF-B antibodies
ChIP-chip analysis of GAGA factor and NELF in Drosophila S2 cells
ORGANISM(S): Drosophila melanogaster 
2008-04-04 | E-MEXP-1547 | biostudies-arrayexpress
TranscriptomicsmiRNA profiling of normal mature B cells
miRNA profiling has been performed on normal mature B cells at different stages of differentiation (naive, germinal center and memory). The main goal is to identify miRNA signatures associated with differentiation stages of normal B cells. Keywords: miRNA, normal mature B cells Cells were isolated...
ORGANISM(S): Homo sapiens 
2010-05-16 | E-GEOD-15144 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation to determine MSL1, MSL3, MOF and H4K16Ac binding in Drosophila SL2 and Kc cells reveals MOF as a key regulator of dosage compensation and gene expression in Drosophila
Genome-wide binding profiles of MSL1, MSL3, MOF and H4K16Ac in Drosophila SL2 and Kc cells.
ORGANISM(S): Drosophila melanogaster 
2008-06-12 | E-MEXP-1508 | biostudies-arrayexpress
TranscriptomicsSingle Cell RNA-Sequencing of RNAi treated Schneider S2 cells
2 million cells were seeded in a 2 ml medium containing 58 µg of total dsRNA. For coRNAi treatments 28 µg of each single dsRNA were used. After treatment cells were left to incubate 4 d at 25 °C, and plates were sealed with Parafilm to avoid medium evaporation. 8 samples were prepared with the follo...
ORGANISM(S): Drosophila melanogaster 
2022-01-03 | E-MTAB-11196 | biostudies-arrayexpress
TranscriptomicsEcdysone signaling and transcript signature in Drosophila cells resistant against methoxyfenozide
Methoxyfenozide (RH-2485) represents the group of non-steroidal ecdysteroid agonists with a dibenzoylhydrazine structure, that are used as novel biorational insecticides in the control of insect pests. Here we report on the selection of Drosophila melanogaster S2 cells for resistance to inhibition o...
ORGANISM(S): Drosophila melanogaster 
2010-11-08 | E-GEOD-17748 | biostudies-arrayexpress
TranscriptomicsRNAi profiling of Drosophila after Nipped-B and Rad21 sister chromatid cohesin proteins in Drosophila ML-DmBG3 cells
Effects of Nipped-B and Rad21 sister chromatid cohesin proteins on gene expression data in ML-DmBG3 cells derived from Drosophila melanogaster larval central nervous system; We examined the effects of Nipped-B and Rad21 knockdown on gene expression in BG3 cells using microarrays that measure over 18...
ORGANISM(S): Drosophila melanogaster 
2009-05-27 | E-GEOD-16152 | biostudies-arrayexpress
GenomicsChIPseq in 159 DNMT TKO mESCs upon p300 inhibition
To test if H3K27Ac contributes to chromatin accessibility at enhancers, we globally reduced H3K27Ac levels by chemical inhibition of the histone acetylase p300 (with the small molecule A-485, final concentration 3 μM). This dataset includes ChIP-seq data for H3K27Ac with spike-in normalization, comp...
ORGANISM(S): Mus musculus 
2026-06-04 | E-MTAB-14916 | biostudies-arrayexpress
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