Sort   by:  
 Page size 
ProteomicsCryo-EM structures of Gid12-bound GID E3 reveal steric blockade as a mechanism inhibiting substrate ubiquitylation
Protein degradation, a major eukaryotic response to cellular signals, is subject to numerous layers of regulation. In yeast, the evolutionarily conserved GID E3 ligase mediates glucose-induced degradation of fructose-1,6-bisphosphatase (Fbp1) and other gluconeogenic enzymes. “GID” is a collection of...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-05-29 | PXD028579 | Pride
Gid E3 ligase complex Crzoem 
ProteomicsDoa10/MARCH6 architecture interconnects E3 ligase activity with lipid-binding transmembrane channel to regulate SQLE
Transmembrane E3 ligases play crucial roles in homeostasis. Much protein and organelle quality control, and metabolic regulation, are determined by ER-resident MARCH6 E3 ligases, including Doa10 in yeast. Here, we present Doa10/MARCH6 structural analysis by cryo-EM and AlphaFold predictions, and a s...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD047499 | Pride
Ubiquitylation E3 ubiquitin ligases March6 Membrane proteins Doa10 Cryo-em Sqle 
ProteomicsProfiling of activated RBR E3 ligases using E2V derived activity-based probes
Structure-based E2 enzyme variants (E2Vs) were used to create biotinylated activity-based probes use as handles for affinity purification-mass spectrometry to identify interacting E3s.
ORGANISM(S): Homo sapiens (Human) 
2026-01-05 | PXD068594 | Pride
E2 enzyme E3 ligase Activity-based probe 
ProteomicsInterconversion between anticipatory and active GID E3 ubiquitin ligase conformations via metabolically-driven substrate receptor assembly
Cells respond to environmental changes by toggling metabolic pathways, preparing for homeostasis, and anticipating future stresses. For example, in Saccharomyces cerevisiae starvation-induced gluconeogenesis is terminated upon glucose availability, a process that involves the multiprotein E3 ligase,...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-10-19 | PXD015396 | Pride
E3 ubiquitin ligase Lc-ms/ms 
ProteomicsPrinciples of isoform-specific targeted protein degradation engaging the C-degron E3 KLHDC2
Targeted protein degradation (TPD) via molecular glues or PROTACs (Proteolysis-targeting chimeras) is an up-and-coming drug modality holding promise to drug the “undrugabbles.” Further expanding the collection of targetable E3 ligases for TPD, we report the discovery of ligands targeting the C-END d...
ORGANISM(S): Homo sapiens (Human) 
2025-01-18 | PXD051581 | Pride
Protac Tpd Ubiquitin Brd3 Klhdc2 
ProteomicsCullin-RING ligases target substrates with geometrically optimized catalytic partners
Cullin-RING ubiquitin ligases (CRLs) control the degradation of a wide landscape of human proteins in combination with ubiquitin-carrying enzymes (UCEs). CRL expansion during evolution is apparent, with a few dozen in yeast that function with a single UCE and as many as 300 in humans that function w...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD043523 | Pride
Ubiquitylation Cullin-ring ligase 
TranscriptomicsE2-RING expansion of the NEDD8 cascade confers specificity to cullin modification
Ubiquitin and ubiquitin-like proteins (UBLs) are directed to targets by cascades of E1, E2, and E3 enzymes. The largest ubiquitin E3 subclass consists of cullin-RING ligases (CRLs), which contain one each of several cullins (CUL1, -2, -3, -4, or -5) and RING proteins (RBX1 or -2). CRLs are activated...
ORGANISM(S): Mus musculus 
2009-03-23 | E-GEOD-14088 | biostudies-arrayexpress
ProteomicsCTLH E3 Ligase Regulates the Degradation of HMG-CoA Synthase 1 Through the Pro/N-end Rule Pathway
Supporting raw MS data for paper (doi: 10.1016/j.molcel.2024.04.026) by Yi S.A. et al, titled "CTLH E3 Ligase Regulates the Degradation of HMG-CoA Synthase 1 Through the Pro/N-end Rule Pathway". Index of RAW files uploaded: - Related to Supplementary Table 1 (HA-9-81-X | WT (1), ATG7KO (2), RB1CC1 ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-11-29 | MSV000090814 | MassIVE
ProteomicsARIH2 is a novel Vif-dependent regulator of CUL5-mediated APOBEC3G degradation in HIV infection
Discovery of novel host-virus interactions leads to a better understanding of mechanisms underlying infection and points to potential therapeutic targets at the interface between virus and host proteins. Recently, global, virus-host interaction networks have been mapped using affinity purification-m...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-29 | MSV000084855 | MassIVE
UnknownIn situ cryo-electron tomography of autophagic structures in S. cerevisiae
Not available
EMPIAR-11166 | biostudies-other
Sort   by:  
 Page size