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ProteomicsThe dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinant
A century ago, influenza A virus (IAV) infection caused the 1918 flu pandemic and killed an estimated 20-40 million people. Pandemic IAV outbreaks occur when strains from animal reservoirs acquire the ability to infect and spread among humans. The molecular details of this species barrier are incomp...
ORGANISM(S): Homo sapiens (Human) Gallus gallus (Chicken) 
2019-11-11 | PXD015475 | Pride
Influenza a virus infection 
ProteomicsThe dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinant
A century ago, influenza A virus (IAV) infection caused the 1918 flu pandemic and killed an estimated 20-40 million people. Pandemic IAV outbreaks occur when strains from animal reservoirs acquire the ability to infect and spread among humans. The molecular details of this species barrier are incomp...
ORGANISM(S): Homo sapiens (Human) 
2019-11-11 | PXD015474 | Pride
Influenza a virus Human Avian Newly synthesized proteins 
ProteomicsInteractome of Nematostella GW182 in HEK293 cells
Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translat...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080764 | MassIVE
ProteomicsProteome wide measurements of protein half-lives in Mycoplasma pneumoniae
We obtained proteome wide measurements of protein half-lives in M. pneumoniae by performing pulse-chase experiments following SILAC methods. We estimated degradation rates of individual proteins by measuring the increase in light protein compared to heavy protein labeling along the growth curve.
ORGANISM(S): Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
2020-11-24 | PXD016386 | Pride
Degradation rates Protein half-life Silac 
ProteomicsIPG Strip-Based Peptide Fractionation for Shotgun Proteomics
This deep proteome dataset of human HEK293 cells was generated using IPG Strip-Based fractionation.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-21 | MSV000080274 | MassIVE
ProteomicsPicky - a simple online method designer for targeted proteomics
Picky is an online method designer for targeted proteomics. To assess the performance of PRM methods designed by Picky we carried out a benchmark experiment. As reference samples we used different amounts of human proteins spiked into 1.4 µg yeast lysate. These sample where analyzed in PRM and DDA m...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2017-08-04 | PXD007039 | Pride
Prm Benchmark Dda 
ProteomicsRPL12 phosphorylation regulates translation during mitosis - pSILAC B cell PRM -
project_description Emerging evidence indicates that heterogeneity in ribosome composition can give rise to specialized functions. Until now, research mainly focused on differences in core ribosomal proteins and associated factors. The impact of posttranslational modifications has not yet been studi...
ORGANISM(S): Mus musculus (Mouse) 
2019-11-28 | PXD010029 | Pride
Prm Polysome proteome profiling B cell Mouse 
TranscriptomicsUnstressed HeLa cells and ELAVL1/HuR knock down conditions: polyA RNA-Seq, small RNA-Seq, and PAR-CLIP
Post-transcriptional gene regulation relies on hundreds of RNA binding proteins (RBPs) but the function of most RBPs is unknown. The human RBP HuR/ELAVL1 is a conserved mRNA stability regulator. We used PAR-CLIP, a method based on RNA-protein crosslinking, to identify transcriptome wide ~26,000 HuR ...
ORGANISM(S): Homo sapiens 
2011-06-29 | E-GEOD-29943 | biostudies-arrayexpress
TranscriptomicsConservation of mRNA and protein expression during development of C. elegans
Spatiotemporal control of gene expression is crucial for development and subject to evolutionary changes. Although proteins are the final product of most genes, the developmental proteome of an animal has not yet been comprehensively defined, and the correlation between RNA and protein abundance dur...
ORGANISM(S): Caenorhabditis elegans 
2014-01-23 | E-GEOD-53359 | biostudies-arrayexpress
TranscriptomicsmRNA composition of IRP1 mRNPs in mouse tissues
Affymetrix microarrays were used to determine the mRNA composition of mRNPs obtained by immunoprecipitation with IRP1 (iron regulatory protein 1). Reference Sanchez at al., 2007 Nat. Protoc. Affymetrix microarrays were used to determine the mRNA composition of mRNPs obtained by immunoprecipitation ...
ORGANISM(S): Mus musculus 
2011-12-09 | E-GEOD-17096 | biostudies-arrayexpress
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