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MetabolomicsIlluminating the dark space of neutral glycosphingolipidome by selective enrichment and profiling at multi-structural levels

Glycosphingolipids (GSLs) are essential components of cell membranes, particularly enriched in the nervous system. Altered molecular distributions of GSLs are increasingly associated with human diseases, emphasizing the significance of lipidomic profiling. Traditional GSL analysis methods are ham...

2024-06-25 | MTBLS10400 | MetaboLights
MetabolomicsMetabolic-engineered probiotic-OMVs as nanovaccine mediating sequential immunomodulation for chronic bone infection immunotherapy

Chronic, drug-resistant bone infection caused by methicillin-resistant Staphylococcus aureus (MRSA) features an immunosuppressive niche enabling persistent infection and impaired bone healing. Specific treatment requires initial antibacterial immune activation against infection, followed by resha...

2026-03-19 | MTBLS14091 | MetaboLights
ProteomicsSequential Phosphoproteomic Enrichment through Complementary Metal-Directed Immobilized Metal Ion Affinity Chromatography
We developed a tip-based sequential IMAC based on different kinds of metal ion for increasing the coverage of phosphoproteome. This approach not only can be used for singly and multiply phosphopeptides separation but also for acidic, basophilic and Pro-directed phosphopeptides separation.
ORGANISM(S): Homo Sapiens (human) 
2016-09-07 | PXD004903 | JPOST Repository
ProteomicsSequential Phosphoproteomic Enrichment by Complementary Metal-directed Immobilized Metal Ion Affinity Chromatography: Case Study on Kinase Substrate Mapping in Human Lung Cancer Tissue
Abstract: Despite recent advances in instrumentation and analytical strategies for identification and quantitation of protein phosphorylation, methodologies to enrich the heterogeneous types of phosphopeptides are critical towards comprehensive mapping of the under-explored phosphoproteome. Taking a...
ORGANISM(S): Homo sapiens (Human) 
2014-01-29 | PXD000314 | Pride
Human lung cancer tissue Sequential imac Phosphoproteomics 
ProteomicsPhosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS
The global phosphoproteome analysis by liquid chromatography-mass spectrometry (LC-MS) has emerged as an important tool to study cell signalling. However, the coverage of phosphoproteome by LC-MS is limited despite various approaches for phosphoprotein enrichment. Studies in literature have exploite...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD050933 | Pride
Affinity chromatography Phosphoproteins Enrichment Lc-ms/ms 
ProteomicsProbing the global kinome and phosphoproteome in Chlamydomonas reinhardtii via sequential enrichment and quantitative proteomics
Identifying dynamic protein phosphorylation events is critical for understanding kinase/phosphatase regulated signaling pathways. To date, protein phosphorylation and kinase expression have been examined independently in photosynthetic organisms. Presented here is a method to study the global kinome...
ORGANISM(S): Chlamydomonas reinhardtii 
2016-10-05 | PXD004681 | Pride
Phosphoproteomics Quantitative label-free proteomics Kinome Mass spectrometry Chlamydomonas reinhardtii 
ProteomicsPhosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS
The global phosphoproteome analysis by liquid chromatography-mass spectrometry (LC-MS) has emerged as an important tool to study cell signalling. However, the coverage of phosphoproteome by LC-MS is limited despite various approaches for phosphoprotein enrichment. Studies in literature have exploite...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Cercopithecus aethiops (Green monkey) (Grivet) Pinus radiata (Monterey pine) Drosophila melanogaster (Fruit fly) Caenorhabditis elegans Dictyostelium discoideum (Slime mold) Cicer arietinum (Chickpea) (Garbanzo) Bos taurus (Bovine) Candida albicans (Yeast) Homo sapiens (Human) Brassica oleracea var. botrytis (Cauliflower) Bombyx mori (Silk moth) Brassica napus (Rape) 
2025-10-30 | PXD057032 | Pride
Affinity chromatography Phosphoproteins Enrichment Lc-ms/ms 
ProteomicsSystematic evaluation of experimental parameters for sensitive phosphoproteomics analysis
The field of phosphoproteomics has been revolutionized in the last years, with enhanced efficiency and sensitivity, now enabling deep phosphoproteomic analysis single-shot from minimal peptide inputs. In this study, we rigorously evaluate the effects of various experimental parameters in automated Z...
ORGANISM(S): Homo sapiens (Human) 
2024-03-26 | PXD045601 | Pride
Phosphoproteomics Evosep Sequential enrichment 
UnknownSequential multiple assignment randomization trials with enrichment design.
Not available
S-EPMC5339073 | biostudies-literature
ModelsSGP2024 - Apoptosis_AgNPs
This model explores the temporal dynamics of silver nanoparticles (AgNPs)-induced apoptosis using dynamic simulations in COPASI. It provides a time-resolved framework for understanding how cellular stress progresses to programmed cell death.
2025-09-08 | MODEL2412210001 | BioModels
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