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Proteomicsdevelopment of the cell wall in alfalfa
In this study, the cell wall proteomes of 3 different regions of alfalfa stems were compared. These three regions correspond to three phases in the stem development. The sequential extraction of cell wall proteins with CaCl2, EGTA and LiCl-complemented buffers was combined with a gel-based proteome ...
ORGANISM(S): Medicago sativa (Alfalfa) 
2019-11-12 | PXD010666 | Pride
Cell wall proteins 2d-dige 
ProteomicsAn improved protocol to study the plant cell wall proteome
Cell wall proteins were extracted from alfalfa stems according to a 3-steps extraction procedure using sequentially CaCl2, EGTA and LiCl-complemented buffers. The efficiency of this protocol for extracting cell wall proteins was compared with the 2 previously published methods optimized for alfalfa...
ORGANISM(S): Medicago sativa (Alfalfa) 
2015-04-14 | PXD001927 | Pride
Lc-msms Stem Proteomics 2d-gel electrophoresis Alfalfa Cell wall 
ProteomicsPathogenic leptospires modulate protein expression and post-translational modifications in response to mammalian host signals
A large body of work has demonstrated that leptospires regulate and modify gene expression in response to environmental cues, as encountered during disease transmission, including changes in temperature, osmolarity, concentration of iron, the presence of serum, and interaction with macrophages. Howe...
ORGANISM(S): Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 
2017-08-31 | PXD006995 | Pride
Dige Proteomics Post-translational modifications Spirochetes Leptospira 
Proteomicsimpact of Cd-exposure on medicago sativa cell wall
The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences. It is unessential and harmful to all life forms. The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure. In the c...
ORGANISM(S): Medicago sativa (Alfalfa) 
2019-06-11 | PXD009670 | Pride
Label-free protein quantification Medicago sativa Lignin Long-term cadmium exposure Cell wall Pectin methylesterase 
ProteomicsInsights In The Molecular Regulation Of Monolignol-derived Product Biosynthesis In The Growing Hemp Hypocotyl
Lignin and lignans are both deriving from the monolignol pathway. Despite the similarity of their building blocks, they fulfil different functions in planta. Lignin strengthens the tissues of the plant, while lignans are involved in plant defence and growth regulation. Their biosyntheses are tuned b...
ORGANISM(S): Cannabis 
2018-01-08 | PXD006605 | Pride
Cannabis sativa Lignin Cell wall Lignan 
TranscriptomicsTranscriptomic comparison of chlorhexidine tolerant and sensitive Salmonella Typhimurium
Transcriptional profiles of a chlorhexidine tolerant Salmonella Typhimurium were compared to its, chlorhexidine sensitive, isogenic progenitor isolate. RNA was extracted from mid-log phase cells from both isolates, without chlorhexidine exposure and following exposure to 1 µg/ ml of chlorhexidine fo...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium 
2015-06-06 | E-GEOD-59566 | biostudies-arrayexpress
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