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MetabolomicsQuantitative profiling of endocannabinoids in lipoproteins by LC–MS/MS
Endocannabinoids belong to a diverse family of endogenous lipid bioregulators acting as physiological ligands of cannabinoid receptor type 1 and cannabinoid receptor type 2 in the central and peripheral nervous system. They are also present in nmol/l concentrations in human blood plasma; however, th...
2015-03-11 | MTBLS163 | MetaboLights
TranscriptomicsTranscription profiling of zebrafish germ layer morphogenesis
In gastrulation, distinct progenitor cell populations are induced and sorted into the three germ layers ectoderm, mesoderm and endoderm. In order to identify genes involved in germ layer specification and morphogenesis, we identified genes differentially expressed between ectodermal and mesendoderma...
ORGANISM(S): Danio rerio 
2005-12-01 | E-MEXP-171 | biostudies-arrayexpress
ProteomicsMS Western, a Method of Multiplexed Absolute Protein Quantification is a Practical Alternative to Western Blotting
Develeopment and application of MS Western method. A new GeLC-MS/MS method is developed for the absolute quantification of proteins.
ORGANISM(S): Bos taurus (Bovine) Danio rerio (Zebrafish) (Brachydanio rerio) Homo sapiens (Human) Oryctolagus cuniculus (Rabbit) Drosophila melanogaster (Fruit fly) Saccharomyces cerevisiae (Baker's yeast) 
2017-12-18 | PXD005654 | Pride
Gelc-ms/ms Absolute quantification 
ProteomicsMedian Based Absolute Quantification using Fully Unlabelled Generic Internal Standard
By reporting molar abundances of proteins, absolute quantification determines their stoichiometry in complexes, pathways or networks and also relates them to abundances of non-protein biomolecules. Typically, absolute quantification relies either on protein- specific isotopically labelled peptide st...
ORGANISM(S): Homo sapiens (Human) Caenorhabditis elegans 
2021-11-30 | PXD026886 | Pride
Absolute quantification 
ProteomicsDrosophila melanogaster eye Proteomics
Absolute (molar) quantification determines proteins stoichiometry in complexes, networks and metabolic pathways. We employed MS Western workflow to determine molar abundances of proteins critical for morphogenesis and phototransduction (PT) in eyes of Drosophila melanogaster using a single chimeric ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2020-07-19 | PXD018001 | Pride
Qconcat Drosophila 
ProteomicsEye proteome of Drosophila melanogaster
We report the proteome composition of the Drosophila eye – a compound organ that is highly enriched in membrane proteins. The fly eye is a popular model to study the physiology of vision by means of genetic, pharmacological, and dietary interference.While the eye transcriptome and development-relate...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2024-08-09 | PXD044845 | Pride
Eye Gelc-ms/ms Proteomics Drosophila melanogaster 
ProteomicsMobilization of cholesterol induces the transition from quiescence to growth in Caenorhabditis elegans through steroid hormone and mTOR signaling
C. elegans complete proteome using Gel LC-MS. We compare the proeome of dauer and L3 larval stages
ORGANISM(S): Caenorhabditis elegans 
2024-05-22 | PXD048087 | Pride
L3 C.elegans Dauer 
ProteomicsLFQ_benchmark_artificial_batch_effect
LFQ benchmark from commercial digests according to Kuharev, Joerg, et al. (2015) Sample A 5%, 30%, 65% E.coli, Yeast, Human; 1ug on column Sample B 20%, 15%, 65% E.coli, Yeast, Human; 0.7ug on column Sample C Average of Sample A and B for Spectral Library by Gas-Phase Fractionationation; 1ug on co...
ORGANISM(S): Escherichia Coli (ncbitaxon:562) Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) 
2022-12-05 | MSV000090832 | MassIVE
ProteomicsDIA_staggered_LFQ_Benchmark_High-Performance_QE-HF_30,90,150minGradient
LFQ Benchmark with samples from commercial digests according to Kuharev, Joerg, et al (2015). Sample A: 5% E.coli, 30 % Yeast, 65% Human, 0.9ug on column. Sample B: 20% E.coli, 15% Yeast, 65% Human, 0.9ug on column. (4 replicates per gradient length, one aliquot per 4 samples from one mastermix pe...
ORGANISM(S): Escherichia Coli (ncbitaxon:562) Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) 
2022-12-05 | MSV000090837 | MassIVE
ProteomicsFastCAT accelerates absolute quantification of proteins by using multiple short non-purified chimeric standards
Absolute (molar) quantification of proteins provides the analytical rationale for system-level modelling of diverse molecular mechanisms. FastCAT method employs multiple short (<50 kDa) stable-isotope labeled chimeric proteins (CPs) composed of concatenated quantotypic (Q-) peptides representing the...
ORGANISM(S): Homo sapiens (Human) 
2022-04-01 | PXD030728 | Pride
Absolute quantification of proteins  ms western  qconcat  targeted quantitative proteomics  cerebrospinal fluid  neurodegeneration  neuroinflammation 
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