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TranscriptomicsPhosphate-independent small RNA sequencing of WT and mir-249 mutant C. elegans
MicroRNAs (miRNA), discovered in C. elegans, are short non-coding RNAs that bind and regulate the expression of target mRNAs in animals and plants. C. elegans miRNAs bind to partially complementary sequences in the 3' UTR of the target mRNA, which results in translational repression through mRNA des...
ORGANISM(S): Caenorhabditis elegans 
2013-08-08 | E-GEOD-41900 | biostudies-arrayexpress
TranscriptomicsDegradome sequencing reveals an endogenous microRNA target in C. elegans
Total poly(A) RNAs from wild-type adult stage worms were subjected to high-throughput sequencing using an Illumina platform to detect RNA cleavage fragment. Wild-type (N2) C. elegans
ORGANISM(S): Caenorhabditis elegans 
2013-02-19 | E-GEOD-44336 | biostudies-arrayexpress
TranscriptomicsHIF-1synergizes with glucocorticoids to promote BFU-E progenitor self-renewal
With the aim of finding small molecules that stimulate erythropoiesis earlier than erythropoietin and that enhance CFU-E production, we studied the mechanism by which glucocorticoids increase CFU-E formation. Using BFU-E and CFU-E progenitors purified by a new technique, we demonstrate that glucocor...
ORGANISM(S): Mus musculus 
2010-12-21 | E-GEOD-26086 | biostudies-arrayexpress
TranscriptomicsIdentification and characterization of microRNAs in pepper from 10 tissue samples by high-throughput sequencing
High-throughput sequencing was employed to identify microRNAs in pepper from 10 tissue samples 10 sample examined
ORGANISM(S): Capsicum annuum 
2013-08-08 | E-GEOD-41654 | biostudies-arrayexpress
TranscriptomicsAnalysis of small RNAs in murine neutrophils by Solexa/Illumina genome analyzer
To examine the effects of microRNAs including miR-223 on murine neutrophil function, small RNAs cloning and sequencing from neutrophils were performed. Neutrophils were isolated from a wild-type mouse and total RNAs were prepared. Small RNAs were cloned and sequenced by Solexa/Illumina genome analy...
ORGANISM(S): Mus musculus 
2008-07-31 | E-GEOD-12074 | biostudies-arrayexpress
TranscriptomicsExpanding the MicroRNA Targeting Code: A Novel Type of Site with Centered Pairing
We present “centered sites,” a class of microRNA target sites that lacks both perfect seed pairing and 3'-compensatory pairing and instead has 11–12 contiguous Watson–Crick pairs to the center of the microRNA. In elevated Mg2+, centered sites impart mRNA cleavage, but in cells, centered sites repre...
ORGANISM(S): Homo sapiens 
2010-06-25 | E-GEOD-22068 | biostudies-arrayexpress
TranscriptomicsAnalysis of HeLa cells after transfection with miR-1, miR-124 and miR-181.
Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124, miR-181 versus control transfected HeLa cells. Results were compared to protein down-regulation at 48 hours measured by SILAC-MS. Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124,...
ORGANISM(S): Homo sapiens 
2010-05-25 | E-GEOD-11968 | biostudies-arrayexpress
TranscriptomicsIdentification and characterization of microRNAs in pepper from fruit sample (3rd stage) by high-throughput sequencing
High-throughput sequencing was employed to identify microRNAs in pepper from fruit sample (3rd stage) 1 sample examined
ORGANISM(S): Capsicum annuum 
2013-05-03 | E-GEOD-42219 | biostudies-arrayexpress
TranscriptomicsIdentification and characterization of conserved microRNAs in pepper by high-throughput sequencing
High-throughput sequencing was employed to identify conserved microRNAs in pepper. 1 sample examined: flower and root in pepper
ORGANISM(S): Capsicum annuum 
2015-05-16 | E-GEOD-37999 | biostudies-arrayexpress
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