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MetabolomicsUntargeted Plasma Lipidomics Identifies Oxidative Stress and Inflammation Signatures Associated with Recurrent Leg Ulcer Status in Sickle Cell Disease

Leg ulcers are among the most burdensome complications of sickle cell disease (SCD), yet the molecular determinants underlying ulcer susceptibility and recurrence remain incompletely characterized. We performed untargeted plasma lipidomics by LC–MS in 129 individuals with SCD from the REDS-III Br...

2025-12-19 | MTBLS13538 | MetaboLights
ProteomicsNontargeted plasma proteomics analysis uncovers candidate biomarkers for renal disease and pulmonary hypertension in patients with sickle cell disease
Fifteen microliters of plasma from sickle cell disease patients, and pooled samples, were diluted with 5% deoxycholate and 10 mM DTT, followed by heating at 80 degC for 30 min, alkylation with 25 mM iodoacteamide and digestion with modified trypsin for 4 h at 37 degC. After acidification, samples we...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-01-19 | MSV000093893 | MassIVE
TranscriptomicsMicroRNA expression profile of platelets in sickle cell disease
Circulating platelets from Sickle cell disease (SCD) patients express distinct gene expression patterns that regulate function. The objective of this study is to identify a role of post-transcriptional regulation of the platelet transcriptional signaling by microRNAs. Comparison of microRNA expressi...
ORGANISM(S): Homo sapiens 
2013-06-20 | E-GEOD-41574 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of whole blood from sickle-cell disease patients using globin mRNA reduction
Room temperature whole blood mRNA stabilization procedures, such as the PAX gene system, are critical for the application of transcriptional analysis to population-based clinical studies. Global transcriptome analysis of whole blood RNA using microarrays has proven to be challenging due to the high ...
ORGANISM(S): Homo sapiens 
2009-06-20 | E-GEOD-16728 | biostudies-arrayexpress
ProteomicsThe proteome of neutrophils in sickle cell disease
Polymorphonuclear neutrophils are key actors in the pathophysiology of sickle cell disease, but specific factors underlying their activation and sustained inflammation are not well documented. In the present study, we investigated the proteome of neutrophils by a label-free global comparative approa...
ORGANISM(S): Homo sapiens (Human) 
2020-11-11 | PXD014457 | Pride
Sickle cell disease Human neutrophils Inflammation Homo sapiens Lfq Orbitrap fusion 
TranscriptomicsTranscription profiling of human pulmonary epithelial cells cultured in plasma from sickle cell disease acute chest syndrome and steady-state patients
Clinical variability in sickle cell disease (SCD) suggests a role for extra-erythrocytic factors in the pathogenesis of vasoocclusion. We hypothesized that one potential factor, endothelial dysfunction, results from induction of phenotypic changes by circulating factors in SCD patients. The database...
ORGANISM(S): Homo sapiens 
2007-08-25 | E-GEOD-1849 | biostudies-arrayexpress
TranscriptomicsmiRNA expression profiles of erythroid cells following hydroxyurea treatment for sickle cell anemia
We hypothesized that miRNA regulation may be invloved in hydroxyurea-mediated fetal hemoglobin induction. Microarray analysis was utilized as an initial screening tool to determine differential miRNA expression in CD71+ erythroid cells comparing cells from control individuals without sickle cell ane...
ORGANISM(S): Homo sapiens 
2011-11-21 | E-GEOD-32035 | biostudies-arrayexpress
GenomicsNHLBI: Genetic modifiers of sickle cell anemia severity and fetal hemoglobin expression in the Cooperative Study of Sickle Cell Disease (CSSCD)

Phenotypic heterogeneity is characteristic of sickle cell anemia, a Mendelian disorder caused by homozygosity for the sickle HBB gene (glu6val). Patients have different rates of hemolysis/vasculopathy and viscosity/vasoocclusion-related complications. These complications account for a s...

phs000366.v1.p1 | EGA
TranscriptomicsPrimary human erythroid progenitor cells SAHA treatment samples
Gene expression profiling was performed on primary human erythroid progenitor cells left untreated or treated with 0.5uM SAHA. The worldwide burden of sickle cell disease is enormous, with over 200,000 infants born with the disease each year in Africa alone. Induction of fetal hemoglobin is a valida...
ORGANISM(S): Homo sapiens 
2010-07-29 | E-GEOD-22367 | biostudies-arrayexpress
ProteomicsDevelopment of pathophysiologically relevant models of sickle cell disease and β-thalassemia for therapeutic studies
β-hemoglobin disorders, such as sickle cell disease (SCD) and β-thalassemia (BT), are the most common inherited monogenic blood disorders globally. Despite decades of research, there are only four FDA-approved medications available for the management of SCD with hydroxyurea (HU) being the most widel...
ORGANISM(S): Homo sapiens (Human) 
2024-02-07 | PXD044642 | Pride
Proteomics Mass spectrometer Sickle cell disease Erythroid progenitor line Crispr/cas9 10-plex tmt label Β-thalassemia 
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