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TranscriptomicsmiRNA expression profiles of erythroid cells following hydroxyurea treatment for sickle cell anemia
We hypothesized that miRNA regulation may be invloved in hydroxyurea-mediated fetal hemoglobin induction. Microarray analysis was utilized as an initial screening tool to determine differential miRNA expression in CD71+ erythroid cells comparing cells from control individuals without sickle cell ane...
ORGANISM(S): Homo sapiens 
2011-11-21 | E-GEOD-32035 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human pulmonary epithelial cells cultured in plasma from sickle cell disease acute chest syndrome and steady-state patients
Clinical variability in sickle cell disease (SCD) suggests a role for extra-erythrocytic factors in the pathogenesis of vasoocclusion. We hypothesized that one potential factor, endothelial dysfunction, results from induction of phenotypic changes by circulating factors in SCD patients. The database...
ORGANISM(S): Homo sapiens 
2007-08-25 | E-GEOD-1849 | biostudies-arrayexpress
ProteomicsNontargeted plasma proteomics analysis uncovers candidate biomarkers for renal disease and pulmonary hypertension in patients with sickle cell disease
Fifteen microliters of plasma from sickle cell disease patients, and pooled samples, were diluted with 5% deoxycholate and 10 mM DTT, followed by heating at 80 degC for 30 min, alkylation with 25 mM iodoacteamide and digestion with modified trypsin for 4 h at 37 degC. After acidification, samples we...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-01-19 | MSV000093893 | MassIVE
TranscriptomicsMicroRNA expression profile of platelets in sickle cell disease
Circulating platelets from Sickle cell disease (SCD) patients express distinct gene expression patterns that regulate function. The objective of this study is to identify a role of post-transcriptional regulation of the platelet transcriptional signaling by microRNAs. Comparison of microRNA expressi...
ORGANISM(S): Homo sapiens 
2013-06-20 | E-GEOD-41574 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of whole blood from sickle-cell disease patients using globin mRNA reduction
Room temperature whole blood mRNA stabilization procedures, such as the PAX gene system, are critical for the application of transcriptional analysis to population-based clinical studies. Global transcriptome analysis of whole blood RNA using microarrays has proven to be challenging due to the high ...
ORGANISM(S): Homo sapiens 
2009-06-20 | E-GEOD-16728 | biostudies-arrayexpress
TranscriptomicsPrimary human erythroid progenitor cells SAHA treatment samples
Gene expression profiling was performed on primary human erythroid progenitor cells left untreated or treated with 0.5uM SAHA. The worldwide burden of sickle cell disease is enormous, with over 200,000 infants born with the disease each year in Africa alone. Induction of fetal hemoglobin is a valida...
ORGANISM(S): Homo sapiens 
2010-07-29 | E-GEOD-22367 | biostudies-arrayexpress
TranscriptomicsPrimary human erythroid progenitor cells HDAC1 and HDAC2 shRNA knockdown samples
Gene expression profiling was performed on primary human erythroid progenitor cells expressing a control shRNA (luciferase), two different HDAC1 shRNAs, and two different HDAC2 shRNAs. The worldwide burden of sickle cell disease is enormous, with over 200,000 infants born with the disease each year ...
ORGANISM(S): Homo sapiens 
2010-07-29 | E-GEOD-22366 | biostudies-arrayexpress
TranscriptomicsThe genomic analysis of erythrocyte microRNA expression in sickle cell diseases
Background Since mature erythrocytes are terminally differentiated cells without nuclei and organelles, it is commonly thought that they do not contain nucleic acids. In this study, we have re-examined this issue by analyzing the transcriptome of a purified population of human mature erythrocytes fr...
ORGANISM(S): Homo sapiens 
2009-04-04 | E-GEOD-11060 | biostudies-arrayexpress
TranscriptomicsGene expression data in Meg-01 cells following transfection with platelet-enriched microRNA-1225-3p
Platelets from sickle cell disease patients have differentially expressed microRNAs as compared to platelets from healthy control subjects. The objective of this study is to overexpress an upregulated miRNA,miR-1225-3p, in MEG01 cells to identify putative targets. MEG-01 cells were transfected with ...
ORGANISM(S): Homo sapiens 
2013-06-20 | E-GEOD-41573 | biostudies-arrayexpress
ProteomicsProteomic analyses of urinary exosomes identify heparanase, cathepsin C, 2-macroglobulin and sarcoplasmic endoplasmic Ca2+ ATPase-3 (SERCA3) as potential biomarkers for early diagnosis of sickle nephropathy
Sickle cell disease-induced nephropathy (SCN) is a leading cause of morbidity and mortality in sickle cell disease (SCD). Early intervention is crucial for mitigating its effects. However, current diagnostic methods rely on non-specific tests and may not detect SCN until renal damage has become irre...
ORGANISM(S): Mus Musculus (mouse) 
2024-02-07 | PXD043401 | JPOST Repository
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