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Dynamic modification of histone proteins plays a key role in regulating gene expression. However, histones themselves can also be dynamic, which potentially affects the stability of histone modifications. To determine the molecular mechanisms of histone turnover we developed a parallel screening met...
ORGANISM(S): Saccharomyces cerevisiae 
Mesenteric, brachial and axilary lymph nodes from 4 C57Bl/6 females or 5 CD27KO females, both groups were 6 weeks old, were extracted. The different lymph nodes were pooled and a single cell suspension was created. The cell suspension from WT or CD27KO mice was stained with anti-TCRd-PE (GL3) and an...
ORGANISM(S): Mus musculus 
Gene expression profiles of "spontaneous" and transplanted topotecan-resistant BRCA1;P53-deficient mammary tumors and their matched untreated controls
ORGANISM(S): Mus musculus 
Human cell cultures (MCF7 and SY5Y) were subjected to volatile anesthetic gasses in an operation theater and monitored for full genome gene expression characteristics to learn more about the time dependent molecular mechanisms that occur during surgery and to find the implications that anesthetics m...
ORGANISM(S): Homo sapiens 
97 triple negative tumors were selected from the fresh-frozen tissue bank of the Netherlands Cancer Institute and gene expression profiles were generated using 35K oligonucleotide microarrays. Human breast carcinomas were snap frozen in liquid nitrogen within one hour after surgery and stored in the...
ORGANISM(S): Homo sapiens 
n=18, 36 arrays, RNA of spheres versus RNA of corresponding parental cell line
ORGANISM(S): Homo sapiens 
BfHL's, spor zonder BfNHL's en BfHL4
ORGANISM(S): Homo sapiens 
Fra1 expression in LM2 cells (MDA-MB-231 derivatives, clone 4173 obtained from J. Massague) was silenced using two independent lentiviral sh-RNA constructs. Gene expresssion levels were compared to those in control cells transduced with empty vector.
ORGANISM(S): Homo sapiens 
165 primary breast carcinomas were selected to study the gene expression profile in relation to the risk of developping local recurrence after breast conserving therapy; in addition 15 recurred tumors were profiled for comparison to their primary tumor.
ORGANISM(S): Homo sapiens 
comparison Cyp3a/Mdr1a/1b ko mice with wild-type mice (both FVB background)
ORGANISM(S): Mus musculus 
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