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K-Ras mutations occur frequently in epithelial cancers. Using shRNAs to deplete K-Ras in lung and pancreatic cancer cell lines harboring K-Ras mutations, two classes were identified—lines that do or do not require K-Ras to maintain viability. Comparing these two classes of cancer cells revealed a g...
ORGANISM(S): Homo sapiens 
RPLC-MS/MS is the present workhorse for bottom-up proteomics-based characterization. However it suffers from limited peak capacity and challenges with respect to detection of small and hydrophilic peptides. CZE-MS/MS offers an orthogonal alternative to RPLC-MS/MS but has limited sensitivity due to l...
ORGANISM(S): Mus musculus (Mouse) 
2020-03-05 | PXD017316 | Pride
Metabolic burden can only be understood if changes in gene expression patterns and corresponding proteins/metabolites of the non-recombinant (WT) host were investigated along with recombinant under similar growth conditions. Intriguing cellular regulation in terms of genes and proteins will be helpf...
ORGANISM(S): Escherichia coli 
2024-07-28 | PXD030126 | Pride
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