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ProteomicsAn organotypic model of human skin simulates the dermonecrotic effects of Naja nigricollis envenoming
Snakebite envenoming is a neglected tropical disease that kills and maims hundreds of thousands annually. Naja nigricollis, the black-necked spitting cobra, has a cytotoxin-rich venom that is able to cause severe dermonecrosis and is not efficiently neutralized by current antivenoms. Here, we introd...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2023-01-11 | PXD033111 | Pride
Dermonecrosis Snakebite envenoming Naja nigricollis Skin organotypic Proteomics Cytotoxin Keratinocyte 3d cell culture Dda Lc-ms/ms 
ProteomicsEssential functions of glycans in human epithelia dissected by a CRISPR/Cas9-engineered human organotypic skin model
The glycome undergoes characteristic changes during histogenesis and organogenesis, but our understanding of the importance of select glycan structures for tissue formation and homeostasis is incomplete. Here, we present a human organotypic platform that allows genetic dissection of cellular glycosy...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD018418 | Pride
Skin Glycoproteomics Glycosylation 
ProteomicsDissecting the functional selectivity of TGFβ signaling pathway components using genome engineered human organotypic skin models
Transforming growth factor β (TGFβ) signaling is essential in cell growth and differentiation. Yet, the role of the individual TGFβ signaling components in human tissue homeostasis and transformation is still incompletely understood. Here we dissected the importance of the core components in the TGF...
ORGANISM(S): Homo sapiens (Human) 
2023-02-09 | PXD024935 | Pride
Keratinocyte Proteomics Smad transcription factor Transforming growth factor beta (tgfβ) Organotypic skin culture Crispr/cas9 Phosphoproteomics 
ProteomicsEMSY expression affects multiple components of skin barrier with relevance to atopic dermatitis
Atopic dermatitis (AD) is a heritable inflammatory disease, characterised by skin barrier dysfunction. Genome-wide association studies (GWAS) have identified molecular targets with relevance for drug development, but the strongest genetic association, FLG, has not yet been successfully targeted in a...
ORGANISM(S): Homo sapiens (Human) 
2019-06-24 | PXD014088 | Pride
Human Skin Proteomics Organotypic culture 
TranscriptomicsThe effects of UVB and the organic osmolyte betaine on rat epidermal keratinocytes in an organotypic culture system
Betaine (trimethylglycine) is a non-toxic, highly water-soluble organic osmolyte widely used in skin care due to its assumed moisturizing and protective properties, but only few studies have addressed its specific effects in skin. Here, the cellular and molecular targets of betaine were analyzed by ...
ORGANISM(S): Rattus norvegicus 
2015-10-26 | E-GEOD-63004 | biostudies-arrayexpress
TranscriptomicsExpression data from human 3D skin models in response to IL-31 treatment
Atopic dermatitis, a chronic inflammatory skin disease with increasing prevalance, is closely associated with skin barrier defects. A cytokine related to disease severity and inhibition of keratinocyte differentiation is IL-31. To identify its molecular targets, IL-31-dependent gene expression was d...
ORGANISM(S): Homo sapiens 
2016-02-29 | E-GEOD-76880 | biostudies-arrayexpress
TranscriptomicsOrganotypic Human Epithelial Neoplasia
Refined cancer models are required to assess the burgeoning number of potential targets for cancer therapeutics within a rapid and clinically relevant context. Here we utilize tumor-associated genetic pathways to transform primary human epithelial cells from epidermis, oropharynx, esophagus, and ce...
ORGANISM(S): Homo sapiens 
2010-09-15 | E-GEOD-22382 | biostudies-arrayexpress
UnknownNovel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models.
Not available
S-EPMC10580606 | biostudies-literature
UnknownProtocol for CRISPR-Cas9 modification of glycosylation in 3D organotypic skin models.
Not available
S-EPMC8403582 | biostudies-literature
UnknownEssential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model.
Not available
S-EPMC7497784 | biostudies-literature
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