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ProteomicsStaphylococcus aureus Mediated Tolerance Induction in Human Monocytes is accompanied with Specific Alterations in Membrane Receptor Expression
Exposure of human monocytes to lipopolysaccharide (LPS) or other pathogen-associated molecular pattern (PAMPs) induces a temporary insensitivity to subsequent LPS challenges, a cellular state called endotoxin tolerance (ET). The tolerant state of monocytes is accompanied by cell surface and other gl...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD036036 | Pride
Hydrazide Staphylococcus aureus Membrane proteins N-glycosylation Glycoproteins Monocyte Tolerance Marker of tolerance Pamp 
ProteomicsGlycoproteomes of naive and LPS stimulated human monocytes and THP-1 cells
CD14+ Monocytes from healthy volunteers were purified by MACS (negative selection) and FACSorting and either left untreated or stimulated for 24h and 48h with LPS. THP-1 cells were stimulated for 4h, 24h and 48h with LPS. Glycoproteins were captured with hydrazide chemistry and tryptic and PNGase F-...
ORGANISM(S): Homo sapiens (Human) 
2017-04-24 | PXD004034 | Pride
Monocytes glycoproteomics 
ProteomicsComparison of proteomic sample preparation and data analysis methods by means of human follicular fluids
In-depth proteome exploration of complex body fluids is a challenging task that requires optimal sample preparation and analysis in order to reach novel and meaningful insights. Analysis of follicular fluids is similarly difficult as that of blood serum due to the ubiquitous presence of several high...
ORGANISM(S): Homo sapiens (Human) 
2018-12-04 | PXD009061 | Pride
Proteomics Data analysis Follicular fluids Sample preparation 
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