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UnknownA Simple Light Isotope Metabolic Labeling (SLIM-labeling) Strategy: A Powerful Tool to Address the Dynamics of Proteome Variations In Vivo.
Not available
S-EPMC5672006 | biostudies-literature
ProteomicsExtending the range of SLIM-labeling applications: from human cell lines in culture to Caenorhabditis elegans whole-organism labeling
The simple light isotope metabolic-labeling technique relies on the in-vivo biosynthesis of amino acids from U-[12C]-labeled molecules provided as the sole carbon source. The incorporation of the resulting U-[12C]-amino acids into proteins presents several key advantages for mass-spectrometry-based ...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Caenorhabditis elegans 
2023-06-23 | PXD028880 | Pride
Auxin Mass spectrometry Bottom-up proteomics Human cell line Essential amino acids Slim metabolic labeling Caenorhabditis elegans 
UnknownExtending the Range of SLIM-Labeling Applications: From Human Cell Lines in Culture to Caenorhabditis elegans Whole-Organism Labeling.
Not available
S-EPMC9990122 | biostudies-literature
ProteomicsNovel insights into quantitative proteomics from an innovative bottom-up Simple Light Isotope Metabolic (bSLIM) labeling data processing strategy
The Simple Light Isotope Metabolic labeling (SLIM-labeling) is an inovative method to quantify proteome variations based on an original in vivo labeling strategy. Heterotrophic cells grown on a U-[12C] sole source of carbon synthesize U-[12C]-amino acids which are incorporated into proteins giving r...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-02-15 | PXD021329 | Pride
Openms Data processing workflow Yeast In vivo metabolic labeling Light carbon isotope Quantitative proteomics 12c Knime 
ProteomicsTraining set - Novel insights into quantitative proteomics from an innovative bottom-up Simple Light Isotope Metabolic (bSLIM) labeling data processing strategy
The Simple Light Isotope Metabolic labeling (SLIM-labeling) is an inovative method to quantify proteome variations based on an original in vivo labeling strategy. Heterotrophic cells grown on a U-[12C] sole source of carbon synthesize U-[12C]-amino acids which are incorporated into proteins giving r...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-02-16 | PXD021502 | Pride
Openms Data processing workflow Yeast In vivo metabolic labeling Light carbon isotope Quantitative proteomics 12c Knime 
UnknownNovel Insights into Quantitative Proteomics from an Innovative Bottom-Up Simple Light Isotope Metabolic (bSLIM) Labeling Data Processing Strategy.
Not available
S-EPMC8459934 | biostudies-literature
TranscriptomicsTranscription profiling of Arabidopsis roots from slim1 and the parental line grown +/-S condition
Plants utilize soil sulfate for production of sulfur-containing amino acids that serve as essential dietary sulfur sources for animals. Despite the global nutritional significance of this fundamental metabolic process in nature, transcription factors regulating the plant sulfur assimilation pathways...
ORGANISM(S): Arabidopsis thaliana 
2008-06-13 | E-GEOD-4455 | biostudies-arrayexpress
ProteomicsImproved MS-based proteomics by the reduction of isotopic composition in vivo
Reducing the isotopic composition of proteins in vivo improves in-depth, high resolution MS-based quantitative proteomics. We used U-[12C]-glucose as the metabolic precursor of all amino acids in yeast. This substantially increased the peptide monoisotopic ion intensity in bottom-up analyses, greatl...
ORGANISM(S): Candida albicans (Yeast) 
2017-08-24 | PXD005364 | Pride
Protein half-life [u-12c]-carbon source Candida albicans Bottom-up ms-based proteomics 
TranscriptomicsGenome-wide binding of Fhl1 and Ifh1 +/- Rapamycin
Array design -Platform: amino-silane coated glass slides (GAPS II, Corning) -S. cerevisiae intergenic regions amplified from S288C genomic DNA (ResGen) using the intergenic region primer oligonucleotides (ResGen) (Harismendy et al. EMBO J. 22(18): 4738-4747, 2003). The primers allow the amplificati...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-05 | E-GEOD-1944 | biostudies-arrayexpress
ProteomicsNovel high-resolution ion mobility mass spectrometry for site-specific quantification of the sirtuin-5 regulated kidney succinylome
Protein post-translational modifications (PTMs) dynamically regulate essential biological and cellular processes. Lysine succinylation changes the amino acid charge, potentially affecting protein structures and functions, and dysregulation of protein succinylation may lead to metabolic disorders. Pr...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2025-10-14 | MSV000099489 | MassIVE
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