Sort   by:  
 Page size 
ProteomicsMS-based sequence of anti-RSV-F monoclonal antibody 131-2a
The Respiratory Syncytial Virus (RSV) Fusion (F) protein is a major target of antiviral antibodies following natural infection and vaccination and responsible for mediating fusion between the viral envelope and the host membrane. The fusion process is driven by a large-scale conformational change in...
ORGANISM(S): Mus musculus (Mouse) 
2025-07-22 | PXD059427 | Pride
Antibody Respiratory syncytial virus De novo 
ProteomicsMS-based sequencing of the anti-FLAG-M2 antibody
We demonstrate a method for direct de novo sequencing of monoclonal IgG from the purified antibody products. The method uses a panel of multiple complementary proteases to generate suitable peptides for de novo sequencing by LC-MS/MS in a bottom-up fashion. Furthermore, we apply a dual fragmentation...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-01-11 | PXD023419 | Pride
Antibody Stepped hcd Ethcd De novo sequencing 
ProteomicsTemplate-based assembly of proteomic short reads for de novo antibody sequencing and repertoire profiling
We developed a software package STITCH (https://github.com/snijderlab/stitch) to perform template-based assembly of de novo peptide reads from antibody samples. As a test case we generated de novo peptide reads from protein G purified whole IgG from COVID-19 patients.
ORGANISM(S): Homo sapiens (Human) 
2022-07-21 | PXD031941 | Pride
De novo  antibody  serum 
ProteomicsGlycan shield of the ebolavirus envelope glycoprotein GP
The envelope glycoprotein GP of the ebolaviruses is essential for host cell attachment and entry. It is also the primary target of the protective and neutralizing antibody response in both natural infection and vaccination. GP is heavily glycosylated with up to 17 predicted N-linked sites, numerous ...
ORGANISM(S): Bundibugyo ebolavirus Zaire ebolavirus 
2022-08-02 | PXD031459 | Pride
Virus  ebola  glycosylation 
ProteomicsN-linked glycosylation of recombinant soluble ectodomain of HKU1 haemagglutinin esterase
Coronaviruses (CoVs) encompass many human pathogens such as HKU-1, OC43, NL63, SARS, MERS and, most recently, nCoV-2019. The spike (S) protein of CoVs has received much attention for its role in host tropism and immunity, but it is becoming increasingly clear that the haemagglutinin esterase (HE) al...
ORGANISM(S): Human coronavirus HKU1 
2020-07-24 | PXD017545 | Pride
Ethcd Structure Glycosylation Coronavirus 
ProteomicsGlycoengineering of hCK and MDCK for influenza A virus propagation
Recent human H3N2 influenza A viruses have evolved to bind elongated glycans terminated with α2,6-linked sialic acids. These extended glycans are of low abundance on cells that are used for the propagation of these viruses, which may explain why it is very difficult to isolate and propagate human H3...
ORGANISM(S): Canis familiaris (Dog) (Canis lupus familiaris) 
2024-01-26 | PXD037175 | Pride
Influenza  glycosylation 
ProteomicsGlycosylation profiling of Influenza A Virus H3 HA
The quest for a universal influenza vaccine holds great promise for mitigating the global burden of influenza-related morbidity and mortality. Despite significant progress, challenges persist in identifying conserved epitopes capable of inducing broadly protective immune responses, understanding the...
ORGANISM(S): Influenza A virus 
2026-01-12 | PXD051492 | Pride
Glycosylation  influenza 
ProteomicsLC-MS/MS EThcD of MERS-CoV and SARS-CoV S N-linked glycopeptides from recombinant spike ectodomains and whole MERS virions
Recent outbreaks of severe acute respiratory syndrome and Middle-East respiratory syndrome along with the threat of a future coronavirus pandemic underscore the importance of finding ways to neutralize these viruses. The trimeric spike transmembrane glycoprotein S mediates entry into host cells and ...
ORGANISM(S): Middle East respiratory syndrome-related coronavirus Severe acute respiratory syndrome-related coronavirus 
2019-05-01 | PXD010494 | Pride
Ethcd Sars N-linked glycosylation Mers 
ProteomicsHCOV-NL63 spike protein glycopeptides
HCoV-NL63 is a coronavirus that can cause severe lower respiratory tract infections requiring hospitalization. Of great interest is understanding the HCoV-NL63 coronavirus spike glycoprotein trimer, which is the conformational machine responsible for entry into host cells and the sole target of neut...
ORGANISM(S): Human coronavirus NL63 
2016-09-27 | PXD004557 | Pride
Ethcd Glycopeptide Virus 
ProteomicsGlycopeptides from recombinant EBV gH, gL and gp42 with EThcD
Soluble ectodomains of Epstein Barr Virus glycoproteins gH/gL and gp42 were expressed in HEK293F cells for structural characterization by cryo electron microscopy. The purified material was additionally analyzed by LC-MS/MS after digestion with trypsin or chymotrypsin to identify N-linked glycosylat...
ORGANISM(S): Human herpesvirus 4 
2018-04-10 | PXD006403 | Pride
Glycopeptide  epstein barr virus 
Sort   by:  
 Page size