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MetabolomicsSericin Enhances Cryopreserved Sperm Quality in Chengde Hornless Black Goats by increasing Glutamine metabolism

Cryopreservation of Chengde polled goat semen plays a critical role in conserving genetic resources, enhancing the utilization efficiency of superior breeding rams, and advancing artificial insemination techniques. However, spermatozoa are vulnerable to oxidative stress during the freezing proces...

2025-08-15 | MTBLS12860 | MetaboLights
TranscriptomicsSperm storage mechanisms in poultry
Turkey sperm lose viability within 8-18 h when stored as liquid semen using current methods and extenders. In contrast, turkey hens maintain viable, fertile sperm in their sperm storage tubules (SST) for 45 or more days following a single insemination. Our long-term objectives are to identify and ch...
ORGANISM(S): Meleagris gallopavo 
2003-03-22 | E-GEOD-351 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of sperm storage organs (spermathecae) from female Anopheles gambiae mosquitoes as virgin and 24 hours after mating
Since Anopheles gambiae female mosquitoes mate only once in their lifetime, and store sperm received from males potentially for weeks, genes and pathways regulated by mating in the sperm storage organ (spermatheca) to preserve sperm function may represent new targets for vector control. The transcri...
ORGANISM(S): Anopheles gambiae 
2014-09-18 | E-MTAB-2429 | biostudies-arrayexpress
TranscriptomicsThe Partitioning of RNAs in sperm
A diverse pool of RNAs remain encapsulated within the transcriptionally and translationally silent spermatozoon. These transcripts persist within the male gamete despite the dramatic reduction in cellular volume achieved through expulsion of the cytoplasm and quite possibly the nucleoplasm. The prec...
ORGANISM(S): Mus musculus 
2015-07-08 | E-GEOD-62874 | biostudies-arrayexpress
GenomicsHigh-resolution mapping of chromatin packaging in mouse embryonic stem cells and sperm
Mammalian embryonic stem (ES) cells and sperm exhibit unusual chromatin packaging that plays important roles in cellular function. Here, we extend a recently developed technique, based on deep paired-end sequencing of lightly digested chromatin, to assess footprints of nucleosomes and other DNA-bind...
ORGANISM(S): Mus musculus 
2014-07-08 | E-GEOD-58101 | biostudies-arrayexpress
TranscriptomicsSpermatozoal mRNA is reproducibly detected in purified D. melanogaster sperm.
It is now well established that mature mammalian spermatozoa carry a population of mRNA molecules, at least some of which are transferred to the oocyte at fertilisation. However, the function of the sperm transcriptome remains largely unclear. To shed light on the evolutionary conservation of this...
ORGANISM(S): Drosophila melanogaster 
2012-04-23 | E-GEOD-33947 | biostudies-arrayexpress
GenomicsSequencing and genome-wide mapping of 146 bp mono-nucleosomal DNA from human and bovine sperm
The phenomenon of nucleosome retention in mammalian sperm chromatin is still not clarified yet. The goal of our study was to characterize the binding sites of sperm nucleosomes in the human and bovine genomes, and through this, to clarify whether nucleosome retention in sperm underlies rules of grea...
ORGANISM(S): Bos taurus 
2014-07-08 | E-GEOD-47843 | biostudies-arrayexpress
ProteomicsProteomic Analysis of Spermathecal Fluid in Amphioctopus fangsiao: Molecular Insights into Long-Term Sperm Storage
Amphioctopus fangsiao is an economically important cephalopod species in northern China. A notable reproductive adaptation of this species is the prolonged storage of sperm in the female spermatheca for up to 8 months post-mating, until oocyte maturation and release. Spermathecal fluid, as a critica...
ORGANISM(S): Amphioctopus Fangsiao 
2025-11-20 | PXD071105 |
ProteomicsThe life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts: heavy-male isotopic labeling to identify female-derived sperm proteins
Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used sex-specific isotopic labeling to identify female-derived proteins th...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-03-11 | PXD027802 | Pride
Lc-msms Female reproductive tract Fertility Sperm Silac Drosophila melanogaster 
ProteomicsThe life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts: heavy-female isotopic labeling to estimate abundances of female-derived sperm protein
Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used sex-specific isotopic labeling to estimate the relative abundance of ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-03-14 | PXD027806 | Pride
Lc-msms Female reproductive tract Fertility Sperm Silac Drosophila melanogaster 
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