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ProteomicsIdentification of Native Cross-links in Bacillus subtilis Spore Coat Proteins
The resistance properties of the bacterial spores are partially due to spore surface proteins, ~30% of which are said to form an insoluble protein fraction. Previous research has also identified a group of spore coat proteins affected by spore maturation, which exhibit an increased level of inter pr...
ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) 
2021-01-09 | MSV000086681 | MassIVE
ProteomicsInduced homogenous sporulation by kinA-overexpression leads to altered Bacillus subtilis spore proteomes and spore properties
Summary To facilitate more accurate spore proteomic analysis, the current study focuses on inducing homogeneous sporulation by overexpressing kinA and assesses the effect of sporulation synchronization on spore resistance, structures, the germination behavior at single-spore level and the proteome. ...
ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) 
2020-04-28 | MSV000085346 | MassIVE
ProteomicsCotL, a new morphogenetic coat protein of Clostridium difficile
The strict anaerobe Clostridium difficile is an important nosocomial enteropathogen that has become the most common cause of antibiotic-associated diarrhea. The oxygen-resistant C. difficile spores play a central role in the infectious cycle, contributing to transmission, infection and recurrence. T...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2019-10-16 | PXD010359 | Pride
Spore coat Shotgun proteomics Q exactive hf Clostridium difficile 
ProteomicsStoichiometry and absolute abundance of proteins in the Bacillus cereus spore insoluble fraction determined using a QconCAT approach
Spores of Bacillus cereus pose a threat to food safety due to their high resistance to the heat or acid treatments commonly used to make food microbially safe. As spores survive these treatments and later resume growth either on foodstuffs or, after ingestion, upon entering the gut they are capable ...
ORGANISM(S): Bacillus cereus 
2018-10-26 | PXD003569 | Pride
Bacillus cereus Qconcat Quantitative proteomics Spores 
ProteomicsClostridium sordellii outer spore proteins
Bacterial spores play an important role in disease initiation, transmission and persistence. The outermost spore layer, the exosporium, is important as it is the first point of contact between the spore and the environment and may be involved in spore adherence, protection and germination. Clostridi...
ORGANISM(S): Paeniclostridium sordellii ATCC 9714 
2018-05-01 | PXD009235 | Pride
Spore Clostridium sordellii Bacteria 
TranscriptomicsYeast spore germination: time course
The process of Saccharomyces cerevisiae spore germination includes breakage of dormancy, morphological changes and resumption of vegetative growth. We have determined the global transcriptional response during the first two hours of spore germination in response to rich growth medium and glucose alo...
ORGANISM(S): Saccharomyces cerevisiae 
2012-12-18 | E-GEOD-29960 | biostudies-arrayexpress
ProteomicsIdentification of Clostdridium difficile Immunoreactive Spore Proteins of the Epidemic strain R20291
Clostridium difficile infections are the leading cause of diarrhea associated to the use of antibiotics. During infection, C. difficile initiates a sporulation cycle leading to the persistence of C. difficile spores in the host and disease dissemination. Nowadays, the development of vaccine and pass...
ORGANISM(S): Peptoclostridium difficile (strain R20291) (Clostridium difficile) 
2018-07-05 | PXD008592 | Pride
Cdi patients Isoelectric focusing Spores Clostridium difficile Immunoblotting Two-dimensional electrophoresis Serologic reactivity 
ProteomicsThe influence of sporulation medium on the spore coat protein composition of Bacillus subtilis spores.
Spores are of high interest to the food and health sectors because of their extreme resistance to harsh conditions especially against heat. Earlier research has shown that spores prepared on solid agar plates have a higher heat resistance than those prepared in a liquid medium. It has also been show...
ORGANISM(S): Bacillus subtilis subsp. subtilis 
2017-09-20 | PXD004473 | Pride
Proteomics Sporulation medium Bacillus Quantitative proteomics Spores 
ProteomicsHigh resolution time-lapse proteomic analysis of sporulation
Bacillus subtilis vegetative cells switch to sporulation upon nutrient limitation. To investigate the proteome changeover during sporulation, a time-lapse proteomic analysis was performed in a cell population that was induced to sporulate synchronously. Here, we are the first to comprehensively inve...
ORGANISM(S): Bacillus Subtilis 
2021-04-03 | MSV000087154 | MassIVE
TranscriptomicsComparison of the expression profiles of the 630E strain and a sigF mutant after 14h of growth
Compartment-specific control of gene expression during Bacillus subtilis sporulation is governed by a cascade of four sigma factors, sigmaF and sigmaG in the forespore and sigmaE and sigmaK in the mother cell. In this work, we combined transcriptional analyses and transcriptional start site mapping ...
ORGANISM(S): [Clostridium] difficile 
2014-02-20 | E-GEOD-43199 | biostudies-arrayexpress
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