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ProteomicsInduced homogenous sporulation by kinA-overexpression leads to altered Bacillus subtilis spore proteomes and spore properties
Summary To facilitate more accurate spore proteomic analysis, the current study focuses on inducing homogeneous sporulation by overexpressing kinA and assesses the effect of sporulation synchronization on spore resistance, structures, the germination behavior at single-spore level and the proteome. ...
ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) 
2020-04-28 | MSV000085346 | MassIVE
ProteomicsIntegrative analysis of proteome and transcriptome dynamics during Bacillus subtilis spore revival
Description Bacillus subtilis forms highly resistant, metabolically inactive spores upon nutrient limitation. These endospores pose challenges to the food and medical sectors. Spores reactivate their metabolism upon contact with germinants through germination and outgrowth, and then develop into ve...
ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) 
2020-04-02 | MSV000085217 | MassIVE
ProteomicsClostridium sordellii outer spore proteins
Bacterial spores play an important role in disease initiation, transmission and persistence. The outermost spore layer, the exosporium, is important as it is the first point of contact between the spore and the environment and may be involved in spore adherence, protection and germination. Clostridi...
ORGANISM(S): Paeniclostridium sordellii ATCC 9714 
2018-05-01 | PXD009235 | Pride
Spore Clostridium sordellii Bacteria 
ProteomicsStoichiometry and absolute abundance of proteins in the Bacillus cereus spore insoluble fraction determined using a QconCAT approach
Spores of Bacillus cereus pose a threat to food safety due to their high resistance to the heat or acid treatments commonly used to make food microbially safe. As spores survive these treatments and later resume growth either on foodstuffs or, after ingestion, upon entering the gut they are capable ...
ORGANISM(S): Bacillus cereus 
2018-10-26 | PXD003569 | Pride
Bacillus cereus Qconcat Quantitative proteomics Spores 
ProteomicsIdentification of Clostdridium difficile Immunoreactive Spore Proteins of the Epidemic strain R20291
Clostridium difficile infections are the leading cause of diarrhea associated to the use of antibiotics. During infection, C. difficile initiates a sporulation cycle leading to the persistence of C. difficile spores in the host and disease dissemination. Nowadays, the development of vaccine and pass...
ORGANISM(S): Peptoclostridium difficile (strain R20291) (Clostridium difficile) 
2018-07-05 | PXD008592 | Pride
Cdi patients Isoelectric focusing Spores Clostridium difficile Immunoblotting Two-dimensional electrophoresis Serologic reactivity 
ProteomicsMolecular physiological characterization of a high heat resistant spore forming Bacillus subtilis food isolate
Bacterial endospores (spores) are among the most resistant living forms on earth. Spores of Bacillus subtilis A163 show extremely high resistance to wet heat compared to spores of laboratory strains. In this study, we found that spores of B. subtilis A163 were indeed very wet heat resistant and rele...
ORGANISM(S): Bacillus Subtilis 
2021-03-21 | MSV000087080 | MassIVE
GenomicsIntergrative analysis of proteome and transcriptome dynamics during Bacillus subtilis spore revival
Intergrative analysis of proteome and transcriptome dynamics during Bacillus subtilis spore revival
PRJNA609969 | ENA
ProteomicsHigh resolution time-lapse proteomic analysis of sporulation
Bacillus subtilis vegetative cells switch to sporulation upon nutrient limitation. To investigate the proteome changeover during sporulation, a time-lapse proteomic analysis was performed in a cell population that was induced to sporulate synchronously. Here, we are the first to comprehensively inve...
ORGANISM(S): Bacillus Subtilis 
2021-04-03 | MSV000087154 | MassIVE
TranscriptomicsIntergrative analysis of proteome and transcriptome dynamics during Bacillus subtilis spore revival
To gain insight into spore germination and outgrowth, the transcriptome changes during Bacillus subtilis spore conversion to vegetative cells were analyzed. The transcriptome analysis also allowed us to trace the different functional groups of genes expressed during this conversion. . Our analysis i...
ORGANISM(S): Bacillus subtilis 
2020-03-04 | GSE146277 | GEO
UnknownAnalysis of the Spore Membrane Proteome in Clostridium perfringens Implicates Cyanophycin in Spore Assembly.
Not available
S-EPMC4886753 | biostudies-literature
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