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TranscriptomicsPARE libraries for the identification of miRNA target genes in Norway spruce
We constructed four PARE libraries for the identification of miRNA target genes in Norway spruce. PARE libraries were constructed from four different tissues
ORGANISM(S): Picea abies 
2015-09-02 | E-GEOD-65248 | biostudies-arrayexpress
ProteomicsProteomics of exovesicle-enriched fraction of Norway spruce
Exovesicle-enriched fraction was isolated from the culture medium of tissue-cultured cells of Norway spruce during extracellular lignin formation and when lignin formation was hindered by scavenging of H2O2 from the culture medium. Proteomic analysis was conducted to investigate the protein cargo of...
ORGANISM(S): Picea Abies (ncbitaxon:3329) 
2023-05-16 | MSV000091958 | MassIVE
ProteomicsArabidopsis and spruce grown at different light intensity - proteins in thylakoids
The aim of the project is to estimate the relative protein content of photosynthetic proteins in thylakoids isolated from Arabidopsis leaves and spruce needles grown at different light intensity (high, normal, low).
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Picea abies 
2022-08-18 | PXD029868 | Pride
Thylakoid membranes Arabidopsis Picea Spruce Proteome 
ProteomicsProteomics of exovesicle-enriched fraction of Norway Spruce, non-EDTA-extracted samples
Exovesicle-enriched fraction was isolated from the culture medium of tissue-cultured cells of Norway spruce during extracellular lignin formation and when lignin formation was hindered by scavenging of H2O2 from the culture medium. Proteomic analysis was conducted to investigate the protein cargo of...
ORGANISM(S): Picea Abies (ncbitaxon:3329) 
2023-06-30 | MSV000092312 | MassIVE
TranscriptomicsGlobal monitoring of autumn gene expression within and among populations of Sitka spruce
Cold acclimation in conifers is a complex process, the timing and extent of which reflects local adaptation and varies widely along latitudinal gradients for many temperate and boreal tree species. In spite of their ecological and economic importance, little is known about the global changes in gene...
ORGANISM(S): Picea sitchensis 
2008-05-31 | E-GEOD-8370 | biostudies-arrayexpress
ProteomicsThylakoids of Picea abies - The unique photosynthetic apparatus of Pinaceae - Analysis of photosynthetic complexes in Norway spruce (Piceaabies)
Analysis of the subunits composition of the thylakoids protein complexes in Picea abies (Norway spruce) by means of two-dimensional large-pore Blue-Native/sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D lpBN/SDS-PAGE) and in-gel tryptic digestion of single spots.
ORGANISM(S): Picea abies 
2019-04-02 | PXD010071 | Pride
Spruce Thylakoids Photosynthesis Picea abies 2d lpbn-sds-page 
TranscriptomicsTranscriptome analysis of constitutive resistance to white pine weevil in Picea glauca-engelmannii (Interior spruce)
White pine weevil is a major pest of conifers in North America, especially for Spruce trees. Constitutive defenses are important in understanding defense mechanisms because they constitute the initial barrier to attacks by weevils and other pests. Resistant and susceptible trees exhibit constitutive...
ORGANISM(S): Picea engelmannii x Picea glauca 
2011-11-28 | E-GEOD-27476 | biostudies-arrayexpress
TranscriptomicsPotential link between biotic defense activation and recalcitrance to induction of somatic embryogenesis in shoot primordia from adult trees of white spruce (Picea glauca)
The goal is to examine the molecular aspects underpinning somatic embryogenesis (SE) within shoot tissues of adult white spruce trees by conducting transcriptome-wide expression profiling of shoot explants taken from responsive and nonresponsive genotypes. One-color analysis of vegetative buds in so...
ORGANISM(S): Picea glauca 
2013-10-17 | E-GEOD-46977 | biostudies-arrayexpress
ProteomicsIdentification of proteins in separated PSII supercomplexes from spruce (P. abies)
To identify protein composition of photosystem II (PSII) supercomplexes separated from spruce seedlings using sucrose gradient centrifugation. These supercomplexes represent mainly so called C2S2 PSII supercomplex as revealed by high-resolution cryo-EM.
ORGANISM(S): Picea abies 
2023-07-19 | PXD035272 | Pride
Orbitrap exploris Light harvest Spruce Protein Photosystem ii 
ProteomicsUnique organization of photosystem II supercomplexes and megacomplexes in Norway spruce.
Photosystem II (PSII) complexes are organized into large supercomplexes with variable amounts of light-harvesting proteins (Lhcb). A typical PSII supercomplex in plants is formed by four trimers of Lhcb proteins (LHCII trimers), which are bound to the PSII core dimer via monomeric antenna proteins. ...
ORGANISM(S): Picea abies 
2021-09-09 | PXD020138 | Pride
Lc-msms Plant Spruce Photosystem Complex Membrane 
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