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MetabolomicsDoes Storage Post Sampling Influence the Stability of Sebum when used for Mass Spectrometry Metabolomics Analysis?

Sebum is a biofluid excreted by sebaceous glands in the skin. In recent years sebum has been shown to contain endogenous metabolites diagnostic of disease, with remarkable results for Parkinson’s Disease. Given that sebum sampling is facile and non-invasive, its potential for use in clinical bioc...

2025-07-15 | MTBLS7946 | MetaboLights
MetabolomicsTranscriptional Shift and Metabolic Adaptations during Leishmania Quiescence Using Stationary Phase and Drug Pressure as Models

Microorganisms can adopt a quiescent physiological condition which acts as a survival strategy under unfavourable conditions. Quiescent cells are characterized by slow or non-proliferation and deep down-regulation of processes related to biosynthesis. Although quiescence has been described mostly...

2022-02-23 | MTBLS4046 | MetaboLights
ProteomicsLarge-scale characterization of drug mechanism of action using proteome-wide thermal shift assays - Revisions
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to characterize compound-dependent changes in thermal stability, the proteome-wide thermal shift ass...
ORGANISM(S): Homo sapiens (Human) 
2025-02-13 | PXD053138 | Pride
Thermal shift assay Proteomics Drug discovery 
ProteomicsIdentifying Functionally Relevant Proteins and Phosphorylation Sites During T Cell Activation Using an Integrated Thermal Shift Assay
In this study, we sought to identify how protein thermal stability integrates with changes in cell signaling and protein abundance. We developed a pipeline to investigate changes in protein abundance and thermal stabilite by combining the protein abundance and thermal shift measurements into the sam...
ORGANISM(S): Mus musculus (Mouse) 
2024-06-17 | PXD050772 | Pride
Phosphoproteomics T cell activation Thermal stability 
ProteomicsLarge-scale characterization of drug-induced changes in thermal stability using proteome-wide thermal shift assays
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to characterize compound-dependent changes in thermal stability, the proteome-wide thermal shift ass...
ORGANISM(S): Homo sapiens (Human) 
2025-02-13 | PXD048009 | Pride
Mechanism of action Drug development 
TranscriptomicsChanges in mRNA Stability Associated with Cold Stress in Arabidopsis Cells
Control of mRNA half-life is a powerful strategy to adjust individual mRNA levels to various stress conditions, because the mRNA degradation rate controls not only the steady-state mRNA level but also the transition speed of mRNA levels. Here, we analyzed mRNA half-life changes in response to cold s...
ORGANISM(S): Arabidopsis thaliana 
2012-12-16 | E-GEOD-31837 | biostudies-arrayexpress
ProteomicsNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
The membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with m...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-20 | PXD037153 | Pride
Rat liver tissue Igepal Micelles Nonionic surfactants Thermal proteome profiling Nonidet-p40 substitute 
ProteomicsProteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling
Despite the growing interest in using chemical genetics in plant research, small-molecule target identification remains a major challenge. The cellular thermal shift assay coupled with high-resolution massspectrometry (CETSA MS) that monitors changes in the thermal stability of proteins caused by th...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-04-04 | PXD030309 | Pride
Brassinosteroids Auxin Chemical genetics Cellular thermal shift assay 
TranscriptomicsYPD to YPGal timecourse
Timecourse analyses (0 to 850 min) of exponentially growing BQS252 yeast after shift from YPD to YPGal galactose medium. Total RNA in vivo labeled by run-on, or cDNA labelling using random decamers included. Genomic DNA also examined. Keywords: other
ORGANISM(S): Saccharomyces cerevisiae 
2004-07-29 | E-GEOD-1002 | biostudies-arrayexpress
UnknownQuantitation of protein-protein interactions by thermal stability shift analysis.
Not available
S-EPMC3189529 | biostudies-literature
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